电针对非酒精性脂肪肝大鼠下丘脑瘦素及瘦素受体表达的影响  

Effect of Electroacupuncture on Hypothalamic Leptin and Leptin Receptor mRNA Expression in Rats with Nonalcoholic Fatty Live Disease

作　　者：白春艳[1] 卓廉士[1] 朱艺[1] 傅燕[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400016

出　　处：《针刺研究》2010年第4期277-280,292,共5页Acupuncture Research

摘　　要：目的:探讨电针治疗非酒精性脂肪肝(NAFLD)的作用机制。方法:将32只SD大鼠随机分为模型组22只及空白组10只。模型组喂养高脂饲料制备NAFLD模型。8周后将模型组大鼠随机分为对照组11只及针刺组11只。针刺组分别电针"丰隆""足三里""太冲"。治疗4周后检测各组血清甘油三酯(TG)、血清胆固醇(TC),观察肝组织病理学变化,用逆转录酶链聚合反应检测下丘脑组织中瘦素(Lep)及其受体mRNA的表达。结果:造模后空白组与模型组比较,大鼠TC、TG显著升高(P<0.01),肝组织出现弥漫性脂肪变。电针4周后,电针组与对照组比较,大鼠TC、TG显著下降,肝组织脂肪变减轻,下丘脑Lep和瘦素受体mRNA表达升高(P<0.05)。结论:电针对NAFLD大鼠有较好的治疗作用,其作用机制可能是通过调节下丘脑瘦素及其受体mRNA的表达实现的。Objective To observe the effects of electroacupuncture （EA） on leptin and leptin receptor（Ob-rb） mRNA expression in the hypothalamus of nonalcoholic fatty liver disease （NAFLD） rats, so as to reveal its underlying mechanism in the treatment of NAFLD inflammation. Methods Thirty-two male SD rats were randomly divided into control group （n = 10）, model group （ n = 11 ） and EA group （n = 11 ）. NAFLD model was established by feeding the animals with high fat diet for 8 weeks. EA （10 Hz, 1.5 V, 30 min） was applied to unilateral ＂Fenglong＂ （ST 40） and ＂Zusanli＂ （ST 36）, once daily for 4 weeks. After the treatment, all the rats were sacrificed for collecting the liver to make pathological observation after HE staining and for taking the hypothalamus tissue out to detect the leptin mRNA and leptin receptor （Ob-rb） mRNA expression by reverse transcription polymerase chain reaction （RT-PCR）. Serum total cholesterol （TC） and triglyeride （TG） contents were detected by chromatometry. Results Compared with the control group, serum TO and TG levels increased significantly in the model group （P〈0.05）. While compared with model group, both TO and TG levels in EA group were reduced considerably （P〈0.05）. In comparison with control group, the expression of hypothalamic leptin mRNA and Ob-rb mRNA was down-regulated remarkably （P〈0.05）, while 4 weeks after EA treatment, both leptin mRNA expression and Ob-rb mRNA expression were up-regulated obviously （ P〈0.05）. Conclusion EA can lower serum TG and TO contents in NAFLD rats, which is closely related to its effects in up-regulating the expression of hypo-thalamic leptin mRNA and Ob-rb mRNA.

关 键 词：电针 非酒精性脂肪肝 下丘脑 瘦素 瘦素受体 

分 类 号：R245.97[医药卫生—针灸推拿学]