尿毒清对阿霉素肾病大鼠肾纤维化的保护作用  

Protective effect of Niaoduqing on the fibrosis in adriamycin nephropathy rats

作　　者：亓敏[1] 王娜[1] 梁素忍[1] 甄军晖[2] 刘春喜[3] 胡昭[1] 

机构地区：[1]山东大学齐鲁医院肾内科,济南250012 [2]山东大学医学院病理科 [3]山东大学教育部与卫生部心血管功能与重构重点实验室

出　　处：《中华肾脏病杂志》2010年第8期629-633,共5页Chinese Journal of Nephrology

基　　金：国家科技支撑计划（2007BAI04B10）

摘　　要：目的 研究尿毒清对阿霉素肾病大鼠肾纤维化的保护作用及其可能机制.方法 雄性SD大鼠48只,随机分为正常对照组、模型组、尿毒清组、贝那普利组,每组12只.采用左侧肾脏切除加尾静脉重复注射阿霉素的方法建立阿霉素肾病模型.术后1周尿毒清组给予尿毒清3 g·kg-1·d-1灌胃,贝那普利组子贝那普利4 mg·kg-1·d-1灌胃.用药4、8周后观察各组大鼠24 h尿蛋白量、血生化及光镜、电镜下肾脏病理变化.应用免疫组化方法测定肾组织骨调素（OPN）、纤连蛋内（FN）、胶原蛋白Ⅳ（COLⅣ）的表达.结果 模犁组大鼠24 h尿蛋白量、BUN、Scr、胆同醇（Cho）、三酰甘油（TG）水平及系膜肾小球系膜基质比（M/G）均显著高于正常对照组（P〈0.01）;血白蛋白（Alb）水平显著低于正常对照组（P〈0.01）;肾组织OPN、FN、COLIV的表达亦显著高于正常对照组（P〈0.01）.尿毒清组24 h尿蛋白量、BUN、Scr、Cho、TG水平及M/G均显著低于模型组（P〈0.01）;肾组织OPN、FN、COLIV表达亦显著低于模型组（P〈0.01）.结论 尿毒清可减少蛋白尿的排泄,纠正脂代谢紊乱,改善肾功能,加强FN、COLIV的降解,抑制肾小球细胞外基质的过度积聚,从而减轻肾脏病理损害,发挥其肾脏保护作用.Objective To investigate the renoprective effect and its possible mechanism of Niaoduqing on the adriamycin（ADR）-induced nephropathy rats. Methods Forty eight male Sprague-Dawley rats were randomly divided into normal control（n=12）, ADR-induced nephropathy（model, n = 12）, Benazepril-treated ADR nephropathy（Benazepril, n=12）and Niaoduqing-treated ADR nephropathy（Niaoduqing, n =12）groups. The rat nephropathy model was established by adriamycin injection and unilateral nephrectomy. The rats were sacrifficed per batch at the 4th and 8th weekend.The pathological change of nephridial tissue, the 24-hour urinary protein excretion and renal function were examined. Immunohistochemistry was used to meassure the expression of fibronection（FN）, collagenⅣ（COLⅣ）, osteopontin（OPN）. Results The 24-hour urinary protein excretion, BUN, Scr, triglyeride（TG）, cholesterol（Cho）in model group were significantly higher than those in normal group（P〈0.01）, as well as more server glomerulosclerosis in kidney were observed in model group than those in control group（P〈0.01）, while the albumin（Alb）was lower （P〈0.01）. Compared with model group, the 24-hour urinary protein excretion, BUN, Scr, TG, Cho were significantly reduced and renal glomerulosclerosis was improved in Niaoduqing group（P〈 0.01）, while the Alb was higher（P〈0.01）. Conclusion Niaoduqing palys an important role in the prevention and treatment for nephropathy.

关 键 词：纤维化 阿柔比星 脂代谢障碍 尿毒清 肾小球肾炎 贝那普利 

分 类 号：R285.5[医药卫生—中药学]