氧化苦参碱通过TGF—β—Smad信号通路调控人增生性瘢痕成纤维细胞增殖及功能  

Regulation of fibroblasts proliferation and function of human hypertrophic scar by Oxymatrine through TGF-β signaling pathway

作　　者：杨平[1] 吴志宏[2] 黄静[2] 王爱丽[2] 徐顺[2] 尤佩芬[2] 周轶群 刘天一[1] 

机构地区：[1]复旦大学附属华东医院整形外科,上海200040 [2]上海市第七人民医院烧伤整形科

出　　处：《中国美容整形外科杂志》2010年第9期557-559,共3页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：上海市卫生局青年科研基金（2007Y22）;上海市浦东新区社会发展局青年基金（PW2007B-6）;上海交通大学医学院自然科学研究基金（2008XJ025）;本课题完成于上海市级医院临床科研资源共享平台（SHDC12007206）

摘　　要：目的研究氧化苦参碱（OM）对人增生性瘢痕（HS）成纤维细胞增殖、α平滑肌肌动蛋白（α—SM-Actin），Ⅰ、Ⅲ型胶原合成的影响，以及对Smad3和Smad7蛋白表达量的影响，探讨OM对HS成纤维细胞抑制作用的可能机制。方法体外常规培养HS成纤维细胞，CCK-8检测不同浓度OM对细胞增殖的抑制情况；real—time PCR检测α-SM—Actin、Ⅰ型和Ⅲ型胶原表达；Western blotting检测Smad3和Smad7蛋白量的改变。结果 OM干预后成纤维细胞增殖明显受抑制，抑制率呈现OM浓度依赖性。OM浓度为400mg／L时，抑制率为53．44％；Ⅰ、Ⅲ型胶原及仅一SM—Aetin的mRNA相对表达量均明显低于无OM干预组细胞；Smad3蛋白表达下降了48．16％，而Smad7蛋白表达量增加了55．24％。结论OM作用于HS成纤维细胞可产生抑制效应，其部分机制是通过TGF—β—Smad信号通路的负性调节，实现对细胞的胶原合成及收缩功能的抑制。Objective To investigate the effects of Oxymatrine on fibroblasts proliferation of human hypertrophic scar, α-smooth muscle actin, the synthesis of collagen Ⅰ, Ⅲ and expressive quantity of smad3 and smad7 protein and explore the possible mechanism about the inhibition of Oxymatrine in hypertrophic sear fibroblasts. Methods The hypertrophic scar fibroblasts were cultured in vitro. The proliferation of the fibroblasts was examined by CCK-8 method. The mRNA synthesis of collagen Ⅰ, Ⅲand α-smooth muscle actin were examined by realtime PCR. Smad3 and smad7 protein were exarrfined by western-blotting method. Results The cellular proliferation was decreased obviously by the treatment of Oxymatrine, and the inhibition radio was concentration dependent. When the concentration was 400 mg/L, the inhibition radio was 53.44% ; the mRNA relative expression of collagen Ⅰ, collagen Ⅲ and a-smooth muscle actin reduced obviously compared with the cells without Oxymatrine treatment. Meanwhile, the expression of smad3 was decreased 48.16% while smad7 was increased 55.24% by the treatment of Oxymatrine. Conclusion The cellular proliferation and functions of hypertrophic scar fibroblasts could all be inhibited by Oxymatrine, and some of the mechanisms were accomplished by blocking the TGF-β- Smad cell signaling pathway.

关 键 词：增生性瘢痕 氧化苦参碱 成纤维细胞 

分 类 号：R619.6[医药卫生—外科学]