支气管哮喘大鼠气道平滑肌细胞表型转变及止喘胶囊的干预作用  

Phenotype Change of Airway Smooth Muscle Cell in Bronchial Asthma Rats and the Intervention of Zhichuan Capsule on It

作　　者：刘芳[1] 方泓[2] 唐斌擎[2] 王丽新[2] 张英兰[2] 吴银根[2] 

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属龙华医院

出　　处：《中国中西医结合杂志》2010年第9期982-985,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家中医药管理局基金资助项目(No.972100)

摘　　要：目的观察支气管哮喘气道平滑肌细胞表型转变及止喘胶囊的干预作用。方法雄性SD大鼠60只随机分为正常对照组、模型组、止喘胶囊组(中药组)、地塞米松组(西药组)、中西医结合组(结合组),每组12只,制备支气管哮喘大鼠模型。于激发第4周用电子显微镜观察气道平滑肌细胞组织形态学改变;分别于激发第2周、第4周检测中小气道smα-actin和sm-MHC蛋白表达。结果电镜下可见模型组气道平滑肌细胞内肌丝稍减少,合成细胞器增多;与正常对照组比较,激发第4周时模型组骨架蛋白sm-MHC和smα-actin表达减少(P<0.05)。西药组平滑肌细胞内肌丝正常,但可见线粒体嵴断裂,基质丢失等缺氧改变;中药组平滑肌细胞内肌丝变化不大,胞内粗面内质网、核糖体未见增多,其高尔基体不发达,而结合组平滑肌细胞同正常对照组;与模型组比较,激发第4周各治疗组smα-actin和sm-MHC表达增强,差异有统计学意义(P<0.05)。结论哮喘大鼠气道平滑肌细胞由收缩表型转变为合成表型,止喘胶囊具有抑制表型转变的作用。Objective To investigate the phenotype change of airway smooth muscle cell（SMC） in bronchial asthma and the effect of Zhichuan Capsule（ZCC） on it.Methods Sixty male SD rats were randomized into 5 groups equally,the normal control group,the model group,and three treated groups treated with ZCC,Western medicine（WM） and ZCC ＋WM respectively.Excepting rats in the control group,they were all made into bronchial asthma model rats and received respective treatment.Histomorphology of the airway SMC was observed by electron microsope at the 4th week,and the expressions of sma-actin and sm-MHC were examined at the 2nd and the 4th week after provocation.Results As compared with the control group,moyfilaments in SMC were lesser,secretive organelles were more and levels of sma-actin and sm-MHC expressions at the 4th week were lower in the model group（P0.05）.While histomorphology of SMC in the WM group was as narmal,but some hypoxia changes were seen,such as the fracture of mitochondria cristae and deplation of matrix; histomorphology of SMC in the ZCC treated group and the ZCC ＋WM treated group were similar to that in the normal control group,and their expressions of sma-actin and sm-MHC were enhanced as compared to those in the model group（P0.05）.Conclusion Airway SMC changes from contractile phenotype to synthetic phenotype in asthma rats,but the progress of change may be inhibited by ZCC.

关 键 词：支气管哮喘 气道平滑肌 表型可塑性 止喘胶囊 

分 类 号：R285.5[医药卫生—中药学]