创伤性脑损伤后脑组织抵抗素rstn基因的表达变化  

Change of gene rstn expression in brain tissues after traumatic brain injury

作　　者：陈大庆[1] 邓应彬 林露阳[1] 朱烈烈[1] 

机构地区：[1]温州医学院附属第二医院急诊创伤外科,325027

出　　处：《中华创伤杂志》2010年第9期845-848,共4页Chinese Journal of Trauma

摘　　要：目的 探讨创伤性脑损伤（TBI）大鼠脑组织抵抗素rstn基因表达的变化特征. 方法选择SD大鼠90只,按随机数字表法分成正常对照组（5只）,假手术组（10只）,轻、中和重度损伤组（每组25只）.应用液压冲击法制作大鼠颅脑损伤模型,并应用RT-PGR方法检测各组大鼠伤后3,6,24,72 h和1,2,4周脑组织rstn基因表达的变化,同时监测损伤中度组大鼠外周血糖浓度变化,观察两者之间的关系. 结果重度损伤组大鼠于伤后24 h,中度损伤组大鼠于伤后72 h,轻度损伤组大鼠于伤后4周rstn基因的表达开始明显上升（P＜0.05）.伤后4周时,所有损伤组海马、丘脑及皮质区域rstn基因表达均升高（P＜0.05）,其中海马区域最高,丘脑区域最低（P＜0.05）.不同区域均存在损伤侧rstn基因表达较高,对侧较低的现象（P＜0.05）.重度损伤组大鼠rstn基因表达升高最明显（P＜0.05）.脑组织rstn基因表达变化与外周血糖浓度呈线性正相关（R=5.32,P=0.03）. 结论 TBI后rstn基因表达明显增加,rstn基因表达的时程变化可能与脑损伤严重程度相关.TBI后rstn基因存在同侧海马区域表达明显升高的组织分布特征.创伤后脑组织rstn基因表达变化与血糖代谢之间存在一定关系.Objective To investigate the change of gene rstn expression in brain tissues following traumatic brain injury （TBI）. Methods A total 90 SD rats were involved in the study and divided into normal control group （5 rats）, sham operation group （10 rats）, mild, moderate and severe trauma groups （25 rats per group）. Rat model was made with sap pressure clash method and RT-PCR assay was employed to detect expression change of gene rstn at 3, 6, 24, 72 h and 1, 2, 4 weeks after TBI. The change of peripheral blood glucose concentration was measured in moderate trauma group to observe its relationship with gene rstn expression in brain tissue. Results Postoperative expression of gene rstn was increased in severe trauma group at 24 hours, in moderate trauma group at 72 hours and in mild trauma group at four weeks （P〈0.05）. The expression of gene rstn was increased in hippocampus, thalamus and cortex of all trauma groups at four weeks postoperatively, when the hippocampus showed the highest expression while the thalamus showed the least （P〈 0.05）. Moreover, the expression of gene rstn showed high level in injury side but low level in contralateral side in different districts （P 〈 0.05）. The expression of gene rstn was increased the most obviously in severe trauma group （P 〈0.05）. Peripheral blood glucose concentration showed a linearity positive correlation with gene rstn expression in brain tissue （R=5.32,P〈0.05）. Conclusions Expression of gene rstn shows obvious increase after TBI, and the time course correlates with the injury severity. The gene rstn expresses the most in the ipsilateral hippocampus. There shows a certain correlation between gene rstn expression and peripheral blood glucose concentration in brain tissues after TBI.

关 键 词：脑损伤 创伤性 rstn基因 时程变化 血糖浓度 

分 类 号：R686[医药卫生—骨科学]