空间诱变长春花代际生物碱含量变异研究  

Variation in Intergenerational Contents of Alkaloids of Catharanthus roseus by Space Mutation

作　　者：于景华[1] 贾雪莹[1] 张学科[1] 唐中华[1] 原树生[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2010年第5期617-622,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金项目资助(30770338)

摘　　要：以经"实践八号"返回式卫星搭载进行空间诱变的长春花种子为材料,研究诱变后代(SP1)及筛选后代(SP2、SP3)长春花叶片文多灵、长春质碱、长春碱等3种吲哚类生物碱的含量变异。结果显示,与留地对照组相比,空间诱变的长春花SP1代形态、生殖等变异增强,叶片生物碱含量变幅加大,变异系数达到留地对照组的2.06倍;经过3轮以长春碱含量为目标的筛选,获得的SP3代4个株系叶片长春碱含量达到300μg.g-1以上,并为留地对照组的3倍以上,株系内变异系数低于15%。上述结果表明,空间环境导致了长春花种子的遗传变异,经过多代筛选,可培育出目的活性物质含量高的优质品种。Using Catharanthus roseus seeds space-mutated by recoverable satellite ＂Shijian-8＂ as material,we studied the content variation of three indole alkaloids（vindoline,catharanthine,vinblastine） in the leaves of C.roseus from the mutated progeny SP1 and selected progeny SP2 and SP3.The result indicated that comparing with the C.roseus cultivated in earth,C.roseus SP1 generation through space mutation had stronger variation ability in morphology and reproduction,and bigger amplitude of the content of alkaloids in the leaves,also the coefficient of variation was 2.06 times of the control group in earth.After three-round selection aiming at the content of vinblastine,the content of vinblastine was above 300 μg·g-1 DW in the leaves of four strains from SP3 generation we gathered,which was more than three times of the control group,and the coefficient of variation in this strain was lower than 15%.The above result showed that spatial environment could change the heredity and variation of the seeds of C.roseus,and after the selection of multi-generation,the variety containing high content of active substance could be cultivated.

关 键 词：长春花 空间诱变 生物碱含量 代际变异 

分 类 号：S331[农业科学—作物遗传育种]