不同剂量胰岛素联合生长激素对内毒素刺激后单核细胞分泌细胞因子的影响  

Effect of different dose insulin combined with growth hormone on the secretion of cytokine in endotoxin-stimulated monocytes

作　　者：陈启仪[1] 虞文魁[1] 龚剑锋[1] 朱维铭[1] 李维勤[1] 李宁[1] 黎介寿[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2010年第5期291-294,共4页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金(30801086);江苏省自然科学基金(BK2007573);教育部博士点新教师基金(200802841005)资助

摘　　要：目的:通过体外培养,观察胰岛素协同生长激素(GH)对内毒素刺激后的单核细胞分泌细胞因子的影响。方法:分离8名健康志愿者外周血单核细胞,用内毒素刺激后,根据GH与胰岛素浓度不同分为五组,即对照组;GH组(GH为100 ng/ml,无胰岛素);GH+胰岛素500μU/ml组、GH+胰岛素1 000μU/ml组和GH+胰岛素2 000μU/ml组。以上各组培养24 h后,采用双抗夹心ELISA法检测单核细胞分泌促炎性细胞因子(TNF-α、IL-1β、IL-6)和抗炎细胞因子(IL-4、IL-1)含量的变化。结果:当培养液中加入100 ng/ml的GH时,TNF-α、IL-1β和IL-6的浓度较对照组明显升高(P<0.01);同时IL-4和IL-10也明显升高(P<0.01)。当不同剂量胰岛素加入培养液后,IL-1β和IL-6浓度均明显低于GH组(P<0.01),并随胰岛素剂量的增加而不断下降;与GH组相比,IL-4的含量在GH+胰岛素1 000μU/ml组和GH+胰岛素2 000μU/ml组中均明显升高(P<0.01);而IL-10仅在GH+胰岛素2 000μU/ml组中升高显著(P<0.01)。结论:GH具有明显促进内毒素刺激后的单核细胞分泌促炎与抗炎细胞因子的效应;而胰岛素联用GH后,促炎细胞因子明显降低,而抗炎细胞因子则进一步升高。Objective：To investigate effect of insulin combined with growth hormone on the secretion of cytokine in endotoxin-stimulated monocytes in vitro.Methods： Peripheral blood monocytes were extracted from 8 healthy volunteers and then stimulated by endotoxin.According to the concentration of insulin and GH,monocytes were divided into five groups： A（without insulin and GH）;B（100 ng/ml GH,no insulin）;C-E（100 ng/ml GH,500,1 000 or 2 000 μU/ml insulin）.After stimulation of lipopolysaccharide and incubation for 24 hours,tumor necrosis factor（TNF-α）,interleukin-1β（IL-1β）,interleukin-6（IL-6）,interleukin-4（IL-4） and interleukin-10（IL-10） were tested by ELISA.Results： Concentrations of all pro-inflammatory and anti-inflammatory cytokine were significantly higher than that of group A after adding GH into culture media（P〈0.05）.However,the concentrations of TNF-α in group D and E were lower than that of group B（P〈0.01） and the secretions of IL-1β and IL-6 in group C,D and E were also lower than that of group B.Compared with group B,insulin could notably increase the secretion of IL-4 in group D and E（P〈0.01）.IL-10 upgraded in group E obviously（P〈0.01）.Conclusions： GH has an effect on endotoxin-stimulated monocytes to secrete pro-inflammatory and anti-inflammatory cytokine and different doses of insulin combined with GH has the opposite effect.

关 键 词：胰岛素 生长激素 脓毒症 单核细胞 细胞因子 

分 类 号：R631[医药卫生—外科学]