HPLC法测定人参叶中人参黄酮苷的质量分数  

Determination of Panasenoside in Leaves of Panax ginseng C.A. Meyer by HPLC

作　　者：李清民[1] 王晓中[2] 周洪玉[2] 魏巍[2] 李绪文[2] 金永日[2] 

机构地区：[1]吉林大学药学院,长春130021 [2]吉林大学化学学院,长春130012

出　　处：《吉林大学学报（理学版）》2010年第5期865-867,共3页Journal of Jilin University:Science Edition

基　　金：吉林省科技厅资助课题(批准号:20070916)

摘　　要：采用高效液相色谱法,测定人参叶中的主要黄酮类成分人参黄酮苷[山柰酚-3-O-β-D-半乳糖(2→1)-β-D-葡萄糖苷]的质量分数.色谱条件:Zorbax Extend C18柱(250mm×4.6mm,5μm),流动相为V(甲醇):V(乙腈):V(体积分数为0.1%的醋酸水溶液)=25:5:70,流速为1.2mL/min,检测波长268nm,色谱柱温度为25℃.结果表明:在进样量0.5～20.0μg范围内线性关系良好,相关系数r=0.9999(n=6);人参黄酮苷的平均回收率为98.5%;RSD=0.64%(n=6)。To establish an HPLC method for the determination of panasenoside [kaempferol-3-O-β-Dglucopyranosyl（1→2）-β-D-galactopyranoside] in the leaves of Panax ginseng C.A.Meyer,the chromatographic separation was performed on a Zorbax Extend C18 column（250 mm × 4.6 mm,5 μm）,with V（methanol）：V（acetonitrile）：V（0.1% acetic acid /water） = 25：5：70 as mobile phase at a rate of 1.2 mL/min and 25 ℃.The detection wavelength was set at 268 nm.The results show that the linearity of panasenoside was in a range of 0.5 ～ 20 μg（r = 0.999 9,n = 6）,and the average recovery and RSD were 98.5% and 0.64% respectively（n = 6）.

关 键 词：人参 人参黄酮苷 高效液相色谱 测定 

分 类 号：O657[理学—分析化学]