全自动细菌分析系统在检测超广谱β-内酰胺酶细菌中的临床应用  

Clinical application of detection of ESBLs with automated becteria analysis system

作　　者：柳振华 

机构地区：[1]山东省聊城市光明眼科医院,252000

出　　处：《中国医学创新》2010年第23期5-7,共3页Medical Innovation of China

摘　　要：目的了解超广谱β-内酰胺酶(ESBLs)细菌在医院分离及分布情况,以利于对ESBLs细菌的监控和治疗。方法用VITEK32型全自动细菌分析系统药敏检测卡GNS-NT进行细菌ESBLs的测定。结果在132株大肠埃希菌、119株肺炎克雷伯菌、60株阴沟肠杆菌中ESBLs的检出率分别为28.8%、19.3%、5.0%,而73株铜绿假单胞菌未检到ESBLs菌;各病区产ESBLs菌的分离率以重症监护(ICU)病房最高(33.6%),其次为慢性病病房和干部病房(均为20.0%),介入科病房(19.6%),呼吸科病房(12.9%),其他病房(3.0%)。产ESBLs菌对亚胺培南全部敏感,对头孢哌酮/舒巴坦末发现有高耐株,中介有23.4%(15/64)。结论 VITEK32型全自动细菌分析系统的GNS-NT卡可正确检测产ESBLs菌,ICU病房是产ESBLs菌的主要来源,对产ESBLs菌的治疗以亚胺培南最佳,其次为高浓度的头孢哌酮/舒巴坦。Objective To investigate the distribution of producing extended -spectrum β-lactamase（ESBLs） bacteria in the hospital for better control and treatment. Methods Producing ESBLs bacteria were detected using GNS - NT susceptibility card of Vitek 32 system. Results Among 132 strains of Escberiehia coli, 119strains of Klebsiella Pneumoniase. 60 strains of Enterebacter cloasas ,producing ESBLs bacteria accounted for 28.8%, 19.3% ,5.0%. No producing ESBLs strains were found in 73 strains of Pseudomenas aerugionsa. The wards isolated rate of ESBLs,in the following order： 33.6% in ICU wards ,20.0% chronic disease and veterans wards, 19.6% interventionwards, 12.9% respiratory system wards,3.0% others. All of the producing ESBLs bacteria wrer susceptible to Imipenem. No strain was highly resistant to cefoperazone/sulbactam but 23.4% was intermediately resistant. Conclusion Producing ESBLs bacteria could be detected accurately with GNS - NT susceptibility card of Vitek 32 system. The main source of producing ESBLs bacteria was ICU. To kill these bacteria, imipenem can be used first,then cefoperazone/sulbaetain.

关 键 词：细菌学技术 微生物敏感性试验 细菌感染 

分 类 号：R446.5[医药卫生—诊断学]