质膜ATP酶介导机械伤害诱导豌豆叶片的防御反应  

Plasma Membrane H^+-ATPase Involved in Mechanical Wounding-Induced Defense Response in Pea Leaves

作　　者：刘艳[1,2] 黄卫东[3] 陈贵林[1] 岳鑫[1] 

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010021 [2]内蒙古农业大学农学院,呼和浩特010019 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国农业科学》2010年第16期3411-3417,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30860180,30871747);内蒙古大学博士后基金项目(207036)

摘　　要：【目的】研究质膜ATP酶(H+-ATPase)在机械胁迫诱导植物防御反应形成中的作用。【方法】通过酶活性生化测定、Western blot检测和电镜细胞化学观测方法,研究机械伤害对豌豆幼苗质膜H+-ATPase活性的影响;同时结合药理学实验,进一步探讨了质膜H+-ATPase与活性氧积累在伤害防御反应中的关系。【结果】机械胁迫能诱导质膜H+-ATPase活性提高,但并未改变该酶蛋白含量;抑制质膜H+-ATPase活性,伤诱导的H2O2累积和苯丙氨酸解氨酶活性提高随之被抑制,活性氧清除剂处理对伤诱导质膜H+-ATPase活性提高没有影响。【结论】质膜H+-ATPase参与机械胁迫诱导防御反应相关的信号传递,该酶可能作用于活性氧信号上游,并通过蛋白磷酸化调节来诱导下游防御反应形成。【Objective】 Effects of plasma membrane H＋-ATPase （PM H＋-ATPase） on defense response to mechanical wounding were investigated. 【Method】 Using pea （Pisum sativum L.）seedlings as materials,treated by mechanical wounding,and using methods of enzymes activity measures,western blot detection and electron microscope cytochemistry,the effects of mechanical wounding on PM H＋-ATPase activities were observed. Furthermore,the relationship between PM H＋-ATPase and accumulation of active oxygen in inducing wounding defense response was studied. 【Result】 Mechanical wounding induced the increasing of PM H＋-ATPase activity,but did not change its content; suppressed PM H＋-ATPase activity,inhibited H2O2 accumulation which has been induced by wounding and also inhibited the phenylalanine ammonia lyase activity＇s increasing,while application of active oxygen scavengers had no effect on the wounding-induced PM H＋-ATPase activity increasing. 【Conclusion】 It is thus suggested that PM H＋-ATPase participated in signal transduction of defense response to mechanical wounding,which acted on the upstream of reactive oxygen signal and induced the formation of defense response on the downstream by protein phosphorylation regulation.

关 键 词：豌豆 机械伤害 质膜ATP酶 防御反应 

分 类 号：S643.3[农业科学—蔬菜学]