宠物源大肠杆菌质粒介导喹诺酮类耐药基因流行性检测  

Detection of Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Pet Animals

作　　者：朱恒乾[1] 廖晓萍[1] 陈朝喜[1] 王秀梅[1] 孙坚[1] 孙迎[1] 李亮[1] 张美君[1] 刘雅红[1] 

机构地区：[1]华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室,广州510642

出　　处：《中国农业科学》2010年第16期3447-3454,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金-广东省政府自然科学基金联合基金重点项目(U0631006);国家自然科学基金项目(30800836);广东省自然科学基金创新团队项目(5200638);教育部创新团队项目(IRT0723)

摘　　要：【目的】对广州分离得到的宠物源(主要是宠物猫、狗)大肠杆菌进行质粒介导喹诺酮类耐药PMQR基因qnr、qepA和aac(6′)-Ib-cr基因的流行性检测。【方法】采用琼脂平板稀释法对164株临床分离的宠物源大肠杆菌进行15种抗菌药物的最小抑菌浓度测定。通过PCR检测qnr、qepA和aac(6′)-Ib-cr基因。建立大肠杆菌基因指纹图谱并对指纹图谱进行分析。【结果】164株宠物源大肠杆菌对12种兽医临床常用抗生素耐药率较高,且表现为多重耐药(5-14耐)。在164株宠物源大肠杆菌中检测到3个菌株同时携带qnrA、qnrB和qnrS基因,其中2株也携带aac(6′)-Ib-cr基因。阳性菌株均能扩增出指纹图谱,具有两个以上PMQR基因的菌株多分布于B型和C型。【结论】广州市宠物源大肠杆菌对临床常用抗菌药物耐药较为严重,检测结果显示喹诺酮类耐药基因在本市宠物医学临床上传播广泛,PMQR基因的产生能力与其基因型有密切的关系。【Objective】 The plasmid-mediated quinolone resistance PMQR genes of qnr,qepA and aac （6＇）-Ib-cr in clinical isolates of Escherichia coli from pet animals （dogs and cats） in Guangzhou city were detected. 【Method】 Susceptibility of 164 strains to 15 antimicrobial agents were determined by agar disc dilution method. All of the 164 strains were screened for the qnr,qepA,aac （6＇）-Ib-cr genes by PCR. Genetic fingerprints of the clinical isolates were established and analyzed. 【Results】 The isolates showed a high rate of resistance to antimicrobial agents widely used in veterinary clinical medicine and most of the them showed multiple-resistance（5 to 14 drugs）. Three of these 164 specimens were detected containing qnrA,qnrB and qnrS genes,and two of which also posses aac（6＇）-Ib-cr gene. Fingerprints could be amplifled from all the positive strains,and strains with more than two PMQR genes were distributed mainly in the B and C gene types. 【Conclusion】 Escherichia coli isolated from pet animals in Guangzhou city showed a high level multiple-resistance to widely used drugs. The result of the research showed a more widespread dissemination of resistance to quinolones in pet animal veterinary clinic of Guangzhou city,and the ability to generate PMQR gene of isolates is closely to their gene-type.

关 键 词：宠物 大肠埃希氏菌 质粒介导 喹诺酮 耐药基因 

分 类 号：S852.61[农业科学—基础兽医学]