软骨细胞—支架复合物修复兔耳廓软骨缺损  

Repair of rabbit auricular defects using chondrocyte-scaffolding constructs

作　　者：刘彦春[1] 王炜[1] 曹谊林[1] 商庆新[1] 夏万尧[1] 钟伟[2] 

机构地区：[1]上海第二医科大学附属第九人民医院整形外科,200011 [2]复旦大学高分子科学系

出　　处：《中华整形烧伤外科杂志》1999年第3期180-182,共3页

基　　金：本课题受上海市领先学科基金资助

摘　　要：目的在有免疫力的动物兔体内探索组织工程化软骨对软骨缺损的修复能力。方法软骨细胞种于经不同物质修饰的聚羟基乙酸支架体外培养后修复兔耳廓软骨缺损,与对照组比较,并从大体及组织学进行评价。结果软骨细胞种于经不同物质修饰的聚羟基乙酸支架体外培养后回植到兔耳廓软骨缺损部位,软骨缺损得到修复,但与正常软骨交界处为纤维组织。注射软骨细胞悬液、以聚羟基乙酸支架修复及空白对照组软骨缺损均未修复。结论软骨细胞种于经不同物质修饰的聚羟基乙酸支架体外培养后在有免疫力的动物兔体内可修复耳廓软骨缺损,但存在着界面愈合问题。Objective To evaluate if the tissue-engineered cartilage can repair the auricular defects in rabbits that have inherent immunity.Methods Rabbit auricular defects were repaired using chondro- cyte-PGA constructs cultured in vitro.The gross and histological comparisons were made between the ex- perimental and the control groups.Results The rabbit auricular defects were repaired with chondrocyte- PGA constructs,leaving fibroid tissue in the interface between the tissue-engineered cartilage and the nor- mal cartilage.Conclusion The auricular defects can be repaired by chondrocyte-PGA in vitro cultured constructs in animals like rabbits that have acquired immunity.The interface healing problems need fur- ther study.

关 键 词：聚羟基乙酸 软骨细胞 修复 耳廓软骨缺损 

分 类 号：R764.91[医药卫生—耳鼻咽喉科]