响应曲面优化醇法提取桑叶黄酮工艺研究  

Optimization of Alcoholic Extraction of Flavonoids from Mulberry Leaves Using Response Surface Methodology

作　　者：章华伟[1] 陈星宇[1] 凌春英[1] 

机构地区：[1]浙江工业大学药学院,杭州310014

出　　处：《氨基酸和生物资源》2010年第3期76-79,86,共5页Amino Acids & Biotic Resources

基　　金：浙江省科技厅项目(2007C12019);浙江省教育厅科研基金(Y200803745)

摘　　要：以含水乙醇为溶剂,在单因素试验基础上,采用响应曲面法优化提取桑叶黄酮工艺条件。结果表明,醇法提取桑叶黄酮的最优工艺条件为:乙醇体积分数71.75%、提取温度67.1℃、料液比23.2:1(醇溶液:桑叶粉,mL:g)、提取时间150 min、提取次数2,桑叶黄酮得率为2.37%。验证试验表明,该优化工艺稳定,与模型预测值相符。On the basis of single factor experiment,response surface methodology（RSM） was applied to optimize extraction of flavonoids from mulberry leaves using alcoholic solution.The results showed that the optimized extraction conditions were 71.75% of ethanol,extraction temperature at 67.1 ℃,ratio of solvent to material of 23.2：1（mL·g^-1）,extraction time of 150 min and extracted two times.Under these extraction conditions,the yield of flavoniods reached at 2.37%,which agreed with the predicted value and indicated the suitability of RSM in optimizing the extraction of flavonoids from mulberry leaves.

关 键 词：桑叶 黄酮 响应曲面法 提取工艺 

分 类 号：R284.2[医药卫生—中药学] S38[医药卫生—中医学]