利用ISSR和RAPD标记构建红麻种质资源分子身份证  

Establishment of Molecular Identity for Kenaf Germplasm Using ISSR and RAPD Markers

作　　者：郑海燕[1] 粟建光[1] 戴志刚[1] 李燕[1] 陈基权[1] 龚友才[1] 

机构地区：[1]中国农业科学院麻类研究所,长沙410205

出　　处：《中国农业科学》2010年第17期3499-3510,共12页Scientia Agricultura Sinica

基　　金：国家"十一五"科技支撑计划项目(2006BAD13B04-2)

摘　　要：【目的】对来源于不同国家和地区的51份红麻栽培种、野生种和近缘种进行遗传分析,构建红麻种质资源分子身份证。【方法】利用ISSR和RAPD标记对不同类型的51份红麻种质资源进行分析,计算其遗传相似系数,利用UPGMA法作聚类图,建立分子身份证。【结果】19个ISSR标记共产生113条条带,其中101条为多态性带,多态性比率(PPB)为89.38%;20个RAPD标记共产生118条条带,其中112条为多态性带,多态性比率(PPB)为94.92%。品种间多态性丰富,结合特征带、特异谱带类型和不同引物组合3种分析方法,可有效建立51份红麻种质资源的特异分子身份证。【结论】材料间遗传多样性较高,有较远的遗传距离和较宽的遗传基础,ISSR和RAPD标记技术可有效用于建立红麻种质资源分子身份证。【Objective】 Fifty-one accessions of kenaf germplasm from different countries and areas,which contain cultivated,wild and related species,were genetically analyzed in order to establish molecular identity of kenaf germplasm.【Method】 Various types of kenaf were used for inter-simple sequence repeat（ISSR） and random amplified polymorphic DNA（RAPD） analysis.The genetic similarity coefficients were estimated and cluster analysis were carried out using UPGMA method.【Result】 Nineteen ISSR primers generated 113 bands,of which 101 were polymorphic and PPB was 89.38%.Twenty RAPD primers generated 118 bands,of which 112 were polymorphic and PPB was 94.92%.The results showed that diversity of kenaf gerplasm was high.Fifty-one specific molecular identities could be established by three methods,which were specific bands,typical band patterns and primer combinations.【Conclusion】 The higher genetic diversity revealed higher genetic distance and wider genetic base.ISSR and RAPD can be used for establishing molecular identity of kenaf germlasm.

关 键 词：红麻 分子标记 种质资源 分子身份证 

分 类 号：S563.5[农业科学—作物学]