用于河南省小麦品种特异性和一致性鉴定的SSR分子标记研究  

Distinctness and Uniformity Evaluation of Wheat (Triticum aestivum) Varieties in Henan by SSR Molecular Analysis

作　　者：韩凤龙[1,2] 李正玲[2] 胡琳[2] 许为钢[2] 

机构地区：[1]南京农业大学农学院/作物遗传与种质创新国家重点实验室,南京210095 [2]河南省农业科学院小麦研究中心,郑州450002

出　　处：《中国农业科学》2010年第18期3698-3704,共7页Scientia Agricultura Sinica

基　　金：国家科技支撑计划(2006BAD13B02-09);河南省重大科技专项(081100110500);河南省农业科学院科研发展基金

摘　　要：【目的】研究可用于小麦品种特异性和一致性鉴定的SSR分子标记特点,筛选出适合河南省小麦品种DUS测定的SSR分子标记。【方法】以18份来源于河南省小麦骨干品种周麦13和周麦16亲缘关系较近的小麦品种为试验材料,选用252对SSR标记进行筛选,分析可用于小麦品种特异性和一致性鉴定的SSR分子标记的特点,对鉴别力较高的标记进行稳定性分析,进而确定出可用于河南省小麦品种DUS测定的骨干引物,并以10份来源于周麦13和周麦16亲缘关系较近的高代品系和41份河南省60年以来的大面积推广品种对骨干引物分辨能力作进一步验证。【结果】SSR分子标记对品种的鉴别力与多态位点数之间存在着极显著的正相关,从252对引物中得到6对特异性和一致性测定表现较好的骨干引物:Xwmc679、Xwmc574、Xgwm497、Xwmc388、Xwmc500、Xgwm637,利用这6对SSR引物可以将本试验所采用的69份小麦材料区别开。【结论】SSR分子标记对品种的鉴别力与多态位点数之间存在着极显著的正相关,Xwmc679、Xwmc574、Xgwm497、Xwmc388、Xwmc500和Xgwm637这6对引物具有较好的品种鉴别力和稳定性,可用于河南省小麦品种特异性和一致性鉴定。Objective The objective of current paper is to study the characteristics of SSR markers used for distinctness and uniformity test of wheat varieties,and to screen suitable SSR molecular markers for DUS test of wheat varieties in Henan Province.Method Eighteen wheat varieties with relative closer genetic relationship to main wheat variety Zhoumai 13 and Zhoumai 16 as experimental materials and 252 pairs of SSR molecular markers were used for distinctness and uniformity analysis in the study.The main primers were screened out by stability analysis of the high discrimination power markers.Furthermore,ten wheat lines derived from Zhoumai 13 and Zhoumai 16 and forty-one widely-used varieties during the past sixty years were used to verify the discrimination power of main primers.Result The results showed that the discrimination power of SSR molecular markers to wheat varieties had significant positive correlations to polymorphism loci.Six main primers with preferable distinctness and uniformity performance（Xwmc679,Xwmc574,Xgwm497,Xwmc388,Xwmc500 and Xgwm637） were screened from 252 SSR primers,which could distinguish completely all the sixty-nine experimental wheat materials used in the study.Conclusion Discrimination power of SSR molecular markers to wheat varieties had significant positive correlation to polymorphism loci.Six SSR primers Xwmc679,Xwmc574,Xgwm497,Xwmc388,Xwmc500 and Xgwm637 showing excellent discrimination power and stability could be used for distinctness and uniformity identification of wheat varieties in Henan Province.

关 键 词：小麦品种 DUS测试 SSR标记 

分 类 号：S512.1[农业科学—作物学]