马铃薯疮痂病新致病种Streptomyces galilaeus致病毒素组分分析  

Analysis of Phytotoxin Ingredients Produced by a New Species,Streptomyces galilaeus,Which Causes Potato Common Scab

作　　者：信净净[1] 于秀梅[2] 赵伟全[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [2]河北农业大学生命科学学院,河北保定071001

出　　处：《中国农业科学》2010年第18期3742-3749,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30700523);国家"863"计划项目(2006AA10A211);现代农业产业技术体系建设专项资金(NYCYTX-15)

摘　　要：【目的】研究马铃薯疮痂病新致病种S.galilaeus菌株CPS-2致病毒素的产生条件、毒素活性和毒素组成情况。【方法】经适宜产毒素培养基和培养时间筛选后大量获取毒素;通过乙酸乙酯萃取、薄层层析、高效液相色谱(HPLC)等技术纯化毒素,所得色谱纯毒素进行质谱分析;采用萝卜幼苗法、小薯片法和幼薯法检测毒素活性。【结果】确定菌株CPS-2的适宜产毒素培养基为燕麦麸皮培养基,产毒素高峰时间为接种后第9天。获得了两个可显著抑制萝卜幼苗生长的毒素组分Ⅱ和Ⅲ,梯度稀释后活性测定结果表明毒素浓度与活性呈正相关,且组分Ⅱ可使小薯片和幼薯组织褐变坏死。质谱分析测定组分Ⅱ的分子量为437.1810,组分Ⅲ的分子量为313.1。【结论】马铃薯疮痂病新致病种S.galilaeus菌株CPS-2可产生致病毒素,至少有两种毒素组分在该病原菌的致病过程中起作用。Objective Phytotoxins produced by the Streptomyces galilaeus strain CPS-2,a new pathogenic species and caused potato scab in China,was studied in order to determine the production condition,activity,and the composition of the phytotoxin.Method Appropriate medium and incubation time for the phytotoxins production of strain CPS-2 were screened.Toxins were purified by using ethyl acetate extract,silica gel thin layer chromatography（TLC）,high performance liquid chromatography（HPLC） methods.The molecular weights of toxin ingredients were determined by mass spectrometry.The toxin activities were examined through radish seedlings test,potato tuber slices test and immature tubers test after each toxin ingredient purification process.Result Oat bran culture medium was selected to be the best medium for toxin production,and the optimal incubation time was 9 d.Two toxin ingredientsⅡand Ⅲ were obtained and showed inhibitory effects on radish seedlings growth.The bioactivity of the two toxin ingredients was correlated to the toxin concentration.The toxin ingredientⅡ could make potato tuber tissue brown and necrosis.The molecular weight of toxin ingredients Ⅱ and Ⅲ were 437.1810 and 313.1,respectively.Conclusion The results showed that the strain CPS-2 of the new plant pathogenic Streptomyces galilaeus could produce phytotoxins.At least two toxin ingredients played an important role in the infection process of the pathogen.

关 键 词：马铃薯疮痂病 链霉菌 致病毒素 毒素组分 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]