甘草中角鲨烯高效液相色谱法测定  

Extraction and HPLC Analysis of the Squalene in Glycyrrhiza uralensis Fisch.

作　　者：梁新华[1,2] 郑彩霞[2] 张风侠[1] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《时珍国医国药》2010年第8期1856-1857,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30600806;30960499);宁夏回族自治区科技攻关项目(No.KGX-07-09-06)

摘　　要：目的对甘草中是否存在角鲨烯进行考察。方法以石油醚为溶剂索氏提取,高效液相色谱法(HPLC)定量分析测试甘草种子、根中角鲨烯。结果甘草种子、甘草根中存在角鲨烯,但含量较低。本实验中所采用的HPLC法测定角鲨烯方法是可行的,确定的色谱条件为:Waters Corporation C18,流动相:乙腈∶甲醇=60∶40,检测波长为210 nm,流速为2.0ml/min,柱温为30℃。结论角鲨烯可以考虑作为甘草中一种新的具有高开发利用价值的物质予以深层次开发利用。Objective To determine squalene in Glycyrrhiza uralensis Fisch by HPLC.MethodsThe oil in root and seed of Glycyrrhiza uralensis Fisch.was extracted by Soxhlets extractor with petrolether,squalene was separated by TLC and HPLC.ResultsThe content of squalene in Glycyrrhiza uralensis Fisch.was low（in olive and palm about 1.16%）.HPLC established in this thesis was suitable to analyze squalene,the HPLC conditions were as follows： column： Waters Corporation C18 column;mobile phase,V（acetonitrile）∶V（methanol）=60∶40;detection wavelength,210nm;flow rate,2ml/min;temperature,30℃.ConclusionThe determination method of aqualene in Glycyrrhiza uralensis Fish was established.

关 键 词：甘草 角鲨烯 提取 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]