β-榄香烯对人胃癌BAC823细胞凋亡及P38MAPK磷酸化的影响  

Effect of β-elemene on Apoptosis and P-P38MAPK of Human Gastric Cancer BAC823 Cells

作　　者：王小晓[1] 瞿延晖[2] 

机构地区：[1]河南中医学院第一附属医院,河南郑州450000 [2]湖南中医药大学,湖南长沙410007

出　　处：《时珍国医国药》2010年第8期1865-1867,共3页Lishizhen Medicine and Materia Medica Research

基　　金：湖南省自然科学基金(No.00JJY2033)

摘　　要：目的探讨P38MAPK信号转导通路在β-榄香烯诱导人胃癌BAC823细胞凋亡过程中调控作用。方法人胃癌BAC823细胞体外传代培养,按空白对照组和药物处理组随机分组,其中药物处理组依药物浓度的不同再分为0.01,0.02,0.04,0.08 mg/ml,0.10及0.16 mg/ml处理组,作用时间为24h,采用流式细胞光度分析术(FCM)检测细胞凋亡率及细胞周期时相分布,同时进行细胞的形态学观察;采用免疫组化法检测P-P38MAPK的表达。结果β-榄香烯可阻滞BAC823细胞于S期并诱导细胞凋亡,但细胞凋亡率并不完全与药物浓度呈正相关。P38MAPK的活化水平随药物浓度的增加而增强。结论β-榄香烯可激活P38MAPK通路,而且此通路也很可能参与了β-榄香烯阻滞细胞周期诱导肿瘤细胞凋亡的过程。Objective To study the effect of β-elemene on signal transduction pathway P38MAPK during apoptosis of cancer cells.MethodsHuman gastric cancer BAC823 cells were cultured in vitro.The cells were divided into two groups including blank control group and drug groups.In terms of concentration,drug groups were divided into 0.01,0.02,0.04,0.08,0.10,and 0.16 mg/ml groups.The effective time was 24h.Apoptotic rate and fraction of the cell cycle phase were measured with flow cytometry（FCM）.At the same time,morphological and adhesive changes of the cells were observed under an inverted microscope.The level of P-P38MAPK was measured with immunocytochemical method.Results1.The result of FCM examination and morphological observation showed that β-elemene could inhibit BAC823 cells at S phase and induce apoptosis.But between the rate of apoptosis and the drug concentration,there was linear correlationship.2.The result of SABC showed that the level of P-P38MAPK was enhanced while the drug concentration increased.Conclusionβ-elemene can activate P38MAPK pathway and the pathway probably takes part in regulating the apoptotic process.

关 键 词：Β-榄香烯 人胃癌BAC823细胞 细胞凋亡 P38MAPK 

分 类 号：R962[医药卫生—药理学]