枇杷叶中齐墩果酸和熊果酸含量的毛细管电泳高频电导法测定  

Determination of Oleanolic Acid and Ursolic Acid Isomers in Folium eriobotryae by Capillary Zone Electrophoresis with High Frequency Conductivity Detection

作　　者：张锋[1] 宋粉云[2] 

机构地区：[1]山西农业大学医院,山西太谷030801 [2]广东药学院,广东广州510224

出　　处：《时珍国医国药》2010年第8期1875-1877,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广东省自然科学基金(No.5002841)

摘　　要：目的建立枇杷叶中齐墩果酸和熊果酸的含量测定方法。方法采用毛细管区带电泳,高频电导检测;未涂层弹性融硅石英毛细管柱55 cm×75μm ID,有效长度46 cm;1.2 mmol.L-1三乙胺-HCl(pH=10.60),0.24 mmol.L-1β-环糊精为运行缓冲液;分离电压12.5 kV;重力进样10 s(高度20 cm)。结果以布洛芬为内标,齐墩果酸、熊果酸线性范围分别为5.1～102.0 g/ml(r=0.999 7)和5.55～111.0 g/ml(r=0.999 3);平均回收率分别为96.0%和97.2%。结论该法简便、准确、快速、重现性好,可用于枇杷叶的质量控制研究。Objective To establish a method for the determination of oleanolic acid and ursolic acid isomers in Folium eriobotryae.MethodsIt was studied by Capillary Zone Electrophoresis with high frequency conductivity detection.The separation was performed on a uncoated fused silica capillary of 55 cm×75 μm ID（46 cm effective length）.1.2 mmol·L-1 Triethylamine（TEA） buffer containing 0.24 mmol·L-1 β-cyclodextrin and pH adjusted to10.60 with Hydrochloric acid was selected for the running buffer.The voltage applied was 12.5 kV and temperature was maintained at 25℃.The sample was injected by gravity（10 s,20 cm）.ResultsThe linear ranges of determination for oleanolic acid and ursolic acid were 5.1～102.0 g/ml（r=0.9997） and 5.55～111.0 g/ml（r=0.999 3）.The average recovery for oleanolic acid and ursolic acid was 96.0%,97.2% respectiviely.ConclusionThe method is reliable,simple,rapid and accurate.The method can be used for quantitative determination of oleanolic acid and ursolic acid isomers in Folium eriobotryae.

关 键 词：毛细管电泳 高频电导检测 枇杷叶 齐墩果酸 熊果酸 

分 类 号：R284.2[医药卫生—中药学]