正交设计法优化野菊花中蒙花苷的提取工艺  

Optimization of Extraction Technology of Linarin from Chrysanthemum indicum L.with Orthogonal Design

作　　者：张明[1] 程文明[1] 李俊[1] 陆维丽[1] 张云静[1] 张群林[1] 

机构地区：[1]安徽医科大学药学院.安徽天然药物活性研究省级重点实验室,安徽合肥230032

出　　处：《时珍国医国药》2010年第8期1882-1883,共2页Lishizhen Medicine and Materia Medica Research

基　　金：安徽省自然科学基金(No090413105);安徽省高等学校省级自然科学研究项目(NoKJ2007B143);安徽医科大学博士科研资助基金(NoXJ200706)

摘　　要：目的为野菊花中主要黄酮成分蒙花苷提取工艺的确定提供实验依据。方法采用正交设计的方法优化提取方案,RP-HPLC法测定蒙花苷的质量分数。结果最终确定优化提取方案为80%乙醇,按8倍药材质量的溶剂量,提取3次,每次2 h。结论提取溶剂量和提取时间对提取效率具有显著影响,应加以控制。优化方案提取效率明显高于正交设计中所有9个实验的提取效率。Objective To provide a basis for the optimum extraction technology of Linarin from Chrysanthemum indicum L..MethodsThe orthogonal design and RP-HPLC method were used.ResultsThe optimum extraction condition was selected as eight times amount of 80% alcohol and refluxing 2 h repeatedly for 3 times.ConclusionThe volume of solvent and extracting time had obvious effect on the extracting efficiency of the three flavonoids in Chrysanthemum indicum L.The optimal method is superior to the nine experiments in orthogonal design.

关 键 词：正交设计 野菊花 蒙花苷 RP-HPLC 提取工艺 

分 类 号：R284[医药卫生—中药学]