6种中药多糖对诱导树突状细胞成熟及刺激T细胞增殖作用的比较  

COMPARISON OF INDUCING DENDRITIC CELL MATURE AND STIMULATING T CELL PROLIFERATION BY SIX TRADITIONAL CHINESE MEDICINE POLYSACCHARIDES

作　　者：董永杰 单铁英[2] 袁征[2] 岳峰 秦志山 杨书良[2] 潘秀兰[2] 

机构地区：[1]河北邵郸市中心医院,邯郸056002 [2]河北工程大学医学院

出　　处：《现代预防医学》2010年第18期3438-3439,3441,共3页Modern Preventive Medicine

摘　　要：[目的]比较6种中药多糖对树突状细胞诱导成熟及刺激T细胞增殖的能力。[方法]通过密度梯度离心法分离人外周血单核细胞,采用细胞因子体外培养7d后,分别加入6种中药多糖,观察细胞的形态变化及用免疫细胞化学法检测其表面标志的表达。通过混合淋巴细胞反应检测细胞提呈抗原的能力。[结果]经6种中药多糖诱导的DC分子表达有明显变化:MHCII+、CD86+、CD83+(P﹤0.05,P﹤0.01),而经RPMI1640培养的DC分子表达与培养前无明显变化。经6种中药多糖诱导的DC均能促进效应T细胞的增殖能力(P﹤0.01)。其中枸杞多糖对上述功能的作用最为明显(P﹤0.05)。[结论]上述6种中药多糖均可诱导DC分化、成熟和刺激T细胞增殖,枸杞多糖与其余5种多糖比较,对上述功能的作用最为明显。[Objective] To compare the capability of six traditional Chinese medicine Polysaccharides on inducing dendritic cell mature and stimulating T cell proliferation.[Methods] Monocytes obtained from human peripheral blood by density guadient centrifugation were cultured in the presence of cytokines in vitro for seven days.Then we cultured them in the presence of six the traditional Chinese medicine polysaccharide.We observed the morphologic changes of DCs and molecule express by immunocytochemical method.The ability of antigenic presentation was examined by mixed lymphology reaction.[Results] DC＇ s molecule express was marked changed by six traditional Chinese medicine Polysaccharides：MHC II＋,CD86＋ and CD83＋（P ﹤0.05,P﹤0.01）,DC＇s molecule express was marked changed by RPMI1640.DC induced by six traditional chinese medicine polysaccharides can reinforce effective T cells proliferation（P﹤0.01）.Lycium bararum polysaccharides was the best among them（P﹤0.05）.[Conclusion] Six traditional Chinese medicine Polysaccharides can induce dendritic cell mature and stimulate T cell proliferation,Lycium bararum polysaccharides is the best among them.

关 键 词：中药多糖 枸杞多糖 树突状细胞 T细胞 

分 类 号：R28[医药卫生—中药学]