两种桑黄发酵产物的抗肿瘤活性对比  

Antitumor Activity of Whole Culture Preparations of Phellinus igniarius and Phellinus vaninii

作　　者：郭佳[1] 宋荣[1] 包海鹰[1] 崔宝凯[2] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]北京林业大学微生物研究所,北京100083

出　　处：《食用菌学报》2010年第2期80-84,共5页Acta Edulis Fungi

基　　金：国家自然科学基金项目"药用木腐真菌‘桑黄’资源及其抗肿瘤活性的研究"(编号:30870013)的部分研究内容

摘　　要：选用H22荷瘤小鼠对火木层孔菌(Phellinus igniarius)和瓦尼木层孔菌(Phellinus vaninii)发酵产物进行抗肿瘤体内试验,测定其对H22荷瘤小鼠抑瘤率、免疫器官指数、免疫因子含量和生存率的影响。结果表明,火木层孔菌和瓦尼木层孔菌发酵产物高、低剂量组(1 000 mg/kg、500 mg/kg)均对肿瘤具有一定抑制作用,其中瓦尼木层孔菌发酵产物低剂量组(500 mg/kg)抑瘤效果最佳,其抑瘤率为58.7%。火木层孔菌和瓦尼木层孔菌发酵产物高、低剂量组均可提高小鼠血清中白细胞介素2(interleukin-2,IL-2)的含量、延长小鼠的生存期,但是对血清中肿瘤坏死因子(tumor necrosis factor-,αTNF-α)含量的影响作用不显著。Whole cultures（mycelium and spent broth） of Phellinus igniarius and Phellinus vaninii grown for 8 days at 30 ℃ on a medium containing sucrose,peptone and mineral salts,were evaporated to dryness in an oven for 24 h at 35 ℃.After grinding to a powder,the solid residues were suspended in physiological saline and the anti-tumor activity of the two preparations（FPPI-P.igniarius,and FPPV-P.vaninii） was determined using H22 tumor-bearing mice as the test model.Both low（500 mg/kg/d） and high（1 000 mg/kg/d） doses of FPPI and FPPV inhibited tumor growth.The inhibitory effect was most pronounced（58.7%） with low doses of FPPV,which was virtually identical to that recorded in the 5-fluorouracil（5-Fu） positive control group.Both low and high doses of FPPI and FPPV also increased interleukin-2（IL-2） levels in test mice and prolonged their survival time.

关 键 词：火木层孔菌 瓦尼木层孔菌 抗肿瘤 

分 类 号：S567.39[农业科学—中草药栽培]