髓复康对脊髓半横断大鼠背根节CGRP表达的影响  

Effect of Chinese Herb(Suifukang)on CGRP Expression in Located Dorsal Root Ganglia in Rats with Spinal Cord Hemitransection

作　　者：韩凤岳[1,2] 唐军民[1,2] 刘卫东[1,2] 唐岩 张平 苏衍萍[1,2] 常超英[1,2] 曲鹏[1,2] 刘恩 

机构地区：[1]中国中医研究院骨伤科研究所 [2]北京医科大学组织胚胎学系

出　　处：《中国骨伤》1999年第3期14-17,共4页China Journal of Orthopaedics and Traumatology

基　　金：国家中医药管理局198年立项课题

摘　　要：目的研究中药髓复康对脊髓损伤后，背根节（ＤＲＧ）神经元降钙素基因相关肽（ＣＧＲＰ）表达的影响。探讨髓复康效应的机理。方法造成下胸髓半横断损伤的Ｗｉｓｔａｒ大鼠模型，用免疫细胞化学法和定量图像分析法检查ＣＧＲＰ表达的变化。结果①脊髓半横断引起同侧首、尾侧各３个ＤＲＧ内，ＣＧＲＰ表达的明显和持续性下调；②损伤后给服髓复康冲剂，可以明显减轻，但不能完全阻止，脊髓损伤诱发的ＣＧＲＰ表达下调；③损伤后给服中药补阳还五汤（或用大剂量的氢化考地松）治疗，不能减轻脊髓损伤诱发的ＣＧＲＰ表达下调；④脊髓损伤后服用髓复康的大鼠，其脊髓损伤区有明显的修复再生。结论结果不仅表明ＣＧＲＰ表达的变化与脊髓组织的损伤和再生有一定的关联；而且也表明，髓复康通过对ＣＧＲＰｍＲＮＡ表达的调制，影响神经元ＤＮＡ的合成，可能是它促进脊髓损伤组织的修复再生的机理之一。此假设还需进一步确认。Objective To study effect of Suifukang on calcitonin gene related peptide (CGRP)expression in dorsal root ganglia(DRG) after spinal cord injury and to inquire into mechanism of Suifukang effect. Methods To make model of righthemitransection at the lower thoracic spinal cord of Wistar rats and to evaluate changes of CGRP expression with immunocytochemistry and quantitative image analysis. Results Spinal cord hemitransection caused a significant downregulation of CGRP expression in ipsilateral 3 DRGs oral and caudal to the injury level respectively.The downregulation of CGRP expression was consistent and irreversible during one month after spinal cord injury.The down-regulation of CGRP expression was evidently relieved but not blocked completely with administration of Suifukang.The downregulation of CGRP expression induced by spinal cord injury could not be relieved with administration of Buyanghuanwutang,or hydrocortisone in a large dose.The injured spinal tissue was repaired and regenerated in rats with administration of Suifukang. Conclusion The results not only suggest that there is relationship among injury,regeneration and CGRP expression in spinal cord,but also indicate that it may be one of mechanisms for promoting repair and regeneration of spinal cord injury that Suifukang modulates CGRPmRNA expression to regulate synthesis of DNA in neurons.However,the suggestions need to be further confirmed.

关 键 词：中药 脊髓再生 背根神经节 CGRP表达 药理 

分 类 号：R286[医药卫生—中药学] R285.5[医药卫生—中医学]