利用18S和ITS序列揭示8种鲇形目鱼类的系统发育  

Phylogenetic Relationship among 8 Common Species of Catfish Based on Ribosome 18S and ITS Sequences

作　　者：杨光[1] 俞菊华[2] 徐跑[1,2,3] 唐永凯[2] 李建林[2] 李红霞[2] 卢中华[3] 阮瑞霞[3] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院淡水渔业研究中心农业部水生动物遗传育种和养殖生物学重点开放实验室,无锡214081 [3]南京农业大学无锡渔业学院,无锡214081

出　　处：《动物学杂志》2010年第4期110-117,共8页Chinese Journal of Zoology

基　　金：国家科技基础条件平台项目(No.2007DKA30470-003);农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金项目(No.BZ2009-08)

摘　　要：为了探讨鲇形目(Siluriformes)鱼类系统发育关系,本研究克隆了黄颡鱼(Pelteobagrus fulvidraco)、长吻(Leiocassis longirostris)、斑鳠(Mystus guttatus)、革胡子鲇(Clarias gariepinus)、鲇鱼(Silurus asotus)和斑点叉尾(Ictalurus punctatus)6种鱼类的18S和两个内转录间隔区(包括全长ITS1-5.8S-ITS2)基因,结合GenBank中双须缺鳍鲇(Kryptopterus bicirrhis)和脂鳍胡鲇(Dinotopterus cunningtoni)的同源序列进行比较分析,结果表明,(1)8种鱼18S的长度为1814～1842bp,同源性达97%以上,5.8S均为157bp,同源性也高达99.36%～100%;(2)8种鱼ITS1长度为335～620bp,其中,黄颡鱼的最长,为618～620bp,斑点叉尾的最短,为335～336bp;ITS2长度为265～459bp,其中,脂鳍胡鲇最长,为459bp,斑点叉尾的最短,约为270bp。ITS1序列的同源性为29.45%～88.21%,其中,革胡子鲇和脂鳍胡鲇同源性最高,鲇鱼和革胡子鲇同源性最低。ITS2序列的同源性为41.59%～94.07%,其中,革胡子鲇和脂鳍胡鲇同源性最高,鲇鱼和革胡子鲇同源性最低;(3)分别以鲤鱼(Cyprinus carpio)18S和ITS为外群,采用NJ法构建18S、ITS系统发育树,结果显示,鲇科与胡鲇科的关系最近,鲿科与这两科关系较远,科与另外3科关系最远。鲿科中属和黄颡鱼属的关系较鳠属更近;胡鲇科的胡鲇属和脂鳍胡鲇属是关系很近的两个属;鲇科的鲇属和缺鳍鲇属是关系较远的两属。In order to study the phylogenetic relationship among different species in the order of Siluriformes, ribosomal 18S sequences and internal transcribed spacer （including complete ITS1,5. 8S and ITS2） sequences of six species Siluriformes （Pelteobagrus fulvidraco,Leiocassis longirostris,Mystus guttatus,Clarias gariepinus, Silurus asotus and Ictalurus punctatus） were analyzed. The homologous sequences of Kryptopterus bicirrhis and Dinotopterus cunningtoni download from GenBank were also analyzed in this paper. The length of 18S sequences of these six species was 1 814-1 842 bp. The homology was over 97% . The length of 5. 8S was 157 bp. The homology was 99. 36%-100% . The length of ITS1 was 335-620 bp,with the P. fulvidraco＇s the longest,and the I. punctatus＇s the shortest; The length of ITS2 was 265-459 bp,with the D. cunningtoni＇s the longest,and the I. punctatus＇s the shortest. The homology of each ITS1 sequence was 29. 45%-88. 21% ,with the highest between C. gariepinus and D. cunningtoni,and the lowest between S. asotus and C. gariepinus. The homology of each ITS2 sequence was 41. 59%-94. 07% ,with the highest between C. gariepinus and D. cunningtoni,and the lowest between S. asotus and C. gariepinus. Phylogenetic tree based on the 18S and ITS sequences of the eight species,including Cyprinus carpio as an outgroup,was constructed by neighbor-joining method （ NJ）. The results showed that the relationship between Siluridae and Clariidae was the closest,and Bagridae had a little further relationship with Siluridae and Clariidae among the 4 families in this paper. Ictaluridae had the furthest relationship with the other three families. In Bagridae,Leiocassis was close with Pelteobagrus,while Mystus was a little further with them. In Clariidae,Clarias was very close with Dinotopterus. In Siluridae, Silurus had a far relationship with Kryptopterus.

关 键 词：鲇形目 18S ITS 系统发育 

分 类 号：Q951.4[生物学—动物学]