培养基中能量底物对猪胚胎发育的影响  

Effect of Energy Substrates on the Development of Porcine Embryos

作　　者：付博[1] 房庆昌[2] 任亮[2] 汪亮[2] 孙洪涛[2] 别墅[2] 马红[1,2] 刘娣 

机构地区：[1]黑龙江省农业科学院,哈尔滨150086 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《动物学杂志》2010年第4期164-170,共7页Chinese Journal of Zoology

基　　金：国家"十一五"科技支撑计划重点项目(No.2008BADB2B02);转基因生物新品种培育重大专项(No.2008ZX08006-003)

摘　　要：旨在探讨丙酮酸和乳酸对猪(Susscrofa)胚胎早期发育的影响,将NCSU-23培养基中的5.56mmol/L葡萄糖替换为0.2mmol/L丙酮酸、5.7mmol/L乳酸,并将此培养基命名为mNCSU-23。根据实验设计,孤雌胚及核移植胚转移到mNCSU-23或NCSU-23中培养。激活第2天统计孤雌胚及核移植胚中的5～8细胞胚胎数。激活第6天统计孤雌胚及核移植胚囊胚形成率及囊胚细胞数。实验结果表明,mNCSU/NCSU处理组的5～8细胞胚胎数及囊胚数显著高于对照组(P<0.05);单纯使用mNCSU培养猪胚胎时,囊胚率最低,发育结果最差(P<0.05)。本研究证实,在体外培养前两天,用乳酸和丙酮酸代替培养基中的葡萄糖对胚胎发育有利。This study aimed to investigate the effects of pyruvate and lactic acid on early development of porcine embryos. The NCSU-23 culture medium was modified by replacing 5. 56 mmol /L glucose with 0. 2 mmol /L pyruvate and 5. 7 mmol /L lactic acid,and the modified medium was called mNCSU-23. Parthenogenetic embryos and nuclear transfer embryos were transferred into either NCSU-23 or mNCSU-23 medium. Embryo development to 5-8 cells was observed 2 days after egg activation,and blastocyst rate and the number of nuclei in the blastocyst were determined on day 6. A higher proportion of the embryos reached 5-8 cells at 48 h and blastocysts on day 6 in mNCSU /NCSU treatment （first 48 h in mNCSU-23 medium and thereafter in NCSU-23 medium） group when compared with the control （NCSU /NCSU,first 48 h in NCSU-23 medium and thereafter also in NCSU-23 medium） group （P〈0. 05）. The rate of blastocyst formation in mNCSU-23 /mNCSU-23 （first 48 h in mNCSU-23 medium and thereafter also in mNCSU-23 medium） group was the lowest （P〈0. 05）. Our results have demonstrated that replacing glucose with pyruvate and lactic acid during the first 48 h of IVC may be beneficial to the development of porcine embryos.

关 键 词：猪 能量底物 孤雌胚 移核胚 

分 类 号：S828[农业科学—畜牧学]