“手十二井穴”放血抗兔实验性脑缺血机理的研究  

Study on Mechanisms of Pricking Blood at "Twelve-well Points of the Hand" in Resisting Experimental Cerebral Ischemia in the Rabbit

作　　者：周国平[1] 邓常青[1] 

机构地区：[1]湖南中医学院,长沙410007

出　　处：《针刺研究》1999年第2期101-104,共4页Acupuncture Research

摘　　要：结扎家兔双侧颈总动脉２小时后，可使其脑组织Ｃａ^（２＋）含量升高（Ｐ＜０．０１），脑组织脂质过氧化物含量上升（Ｐ＜０．０５），脑组织 ｃＡＭＰ含量升高（Ｐ＜０．０５），而缺血后脑组织 ＳＯＤ活性和ＧＳＨ－Ｐｘ活性均明显降低（Ｐ＜０．０１和 Ｐ＜０．０５）。提示脑缺血后脑细胞内 Ｃａ^（２＋）超负荷，自由基产生增加。在缺血同时对家兔施行“十二井穴”放血，可使脑组织Ｃａ^（２＋）含量下降（Ｐ＜０，０５），ＭＤＡ含量下降（Ｐ＜０．０５），ｃＡＭＰ含量降低（Ｐ＜０．０５），而 ＳＯＤ活性则明显升高（Ｐ＜０．０１）。结果提示，“手十二井穴”放血可对抗脑缺血后细胞内Ｃａ^（２＋）超负荷，防止氧自由基的毒副作用。In the present study, the effect of pricking blood of 'twelve-well points of the hand' in resisting cerebral ischemia was observed in 31 rabbits. These rabbits were randomly divided into control group (n = 10 ), ischemia group (n = 11 ) and ischemia plus blood letting group (n = 10). Results showed that two hours after occlusion of rabbit bilateral common carotid arteries, contents of Ca^(2+), lipid peroxide (LPO) and cAMP of the brain tissues all increased significantly (P < 0. 05), but the activity of both superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) lowered evidently (P < 0. 01, 0. 05), indicating overload of intracellular Ca^(2+) and increase of oxygen free radical. After bloodletting of 'twelve-well points', contents of Ca^(2+), malonyldialdehyde (MDA) and cAMP lowered sig- nificantly (P < 0. 05 ), while the activity of SOD increased considerably (P < 0. 01 ). Results suggested that blood-letting of 'twelve-well points' could resist cerebral ischemia-induced overload of intracellular Ca^(2+) of the brain tissues and prevented the toxic action of oxygen free radical.

关 键 词：十二井穴 脑缺血 氧自由基 环核苷酸 针刺 放血 

分 类 号：R246.6[医药卫生—针灸推拿学]