真菌疏水蛋白固定化酶制备胆碱生物传感器  

Fabrication of choline biosensor based on fungus hydrophobins immobilized enzyme

作　　者：王新胜[1] 赵紫霞[2] 王会才[1] 王艳艳[1] 秦霞[1] 陈强[1] 

机构地区：[1]南开大学生命科学学学院生物活性材料教育部重点实验室,天津300071 [2]中国水产科学研究院水产生物应用基因组研究中心,北京100141

出　　处：《传感器与微系统》2010年第9期141-144,共4页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(30772746;30870665);天津市自然科学基金资助项目(08JCZDJC20600)

摘　　要：以Ⅱ型疏水蛋白(HFBI)为胆碱氧化酶(ChOx)固定化基质修饰金电极构建新型电流型胆碱生物传感器。对疏水蛋白修饰电极的电化学特征进行分析,讨论了HFBI自组装膜在不同pH值下的稳定性。结果表明:该传感器对胆碱的线性响应范围为0.01～1.0 mmol/L;灵敏度为2184.06 nA.mmol.L-1.cm-2;响应时间小于6 s;检出限为0.01 mmol/L(信噪比=3)。HFBI自组装薄膜修饰的酶电极高效地保持了固定化ChOx的活性,表现出良好的抗干扰性能和较长的使用寿命,有望为传感器进一步研究和其他表面功能化研究提供一种具有高度生物相容性的电活性生物固定化基质。A novel amperometric choline biosensor is fabricated with choline oxidase （ChOx） immobilized on the surface of a class Ⅱ hydrophobin modified gold electrode. The electrochemistry characteristics of sensor have been analyzed in detail and the stability of HFBI self-assembled film at different pH was investigated. Results showed that the sensor offered a wide linear range from 0.01 to 1 mmol/L, high sensitivity of 2184.06nA·mmol·L^-1·cm^-2, rapid response time within 6 s and detection limit of 0.01 mmol/L choline （ signal-to-noise ratio =3 ）. The HFBI self-assembled film modified electrode kept high electroactivity of immobilization ChOx and well interference immunity and long lifetime. This work provided a promising new immobilization matrix with high biocompatibility and adequate electroactivity for further research on biosensing and other surface functionalized.

关 键 词：疏水蛋白 胆碱氧化酶 生物传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]