转基因玉米MON89034定性PCR检测技术研究  

Event-specific Qualitative PCR Method for Detection of Transgenic Maize MON89034

作　　者：李飞武[1] 董立明[1] 李葱葱[1] 闫伟[1] 邵改革[1] 赵宁[1] 张明[1] 

机构地区：[1]吉林省农业科学院,农业部转基因植物环境安全监督检验测试中心,长春130033

出　　处：《农产食品科技》2010年第3期22-25,共4页

基　　金：国家转基因生物新品种培育重大专项课题（2008ZX08012-001）.

摘　　要：根据转基因抗虫玉米MON89034外源插入片段3′端与植物基因组连接区序列设计特异性引物，建立MON89034玉米转化体特异性定性PCR检测方法，预期产物大小为493bp。对该方法进行特异性和灵敏度测试，结果显示：该方法能够特异性检测出MON89034玉米转化体，以100ngDNA为模板，该方法的检测灵敏度达到0．1％。复合PCR检测结果还表明，在同一PCR反应管中可实现zSSIIb基因和MON89034玉米特异性片段的同时检测。The specific primers were designed based on the 3′-junction sequences of the exogenous integrant of MON89034 maize, and amplification products of 493bp were obtained. The specificity and sensitivity of this method were tested. The results showed that MON89034 can be distinguished specifically from other GM and non-GM crops by using this method, and the limit of detection is up to 0.1%. In addition, a multiplexed PCR reaction was developed by which specific fragment of MON89034 and one fragment of maize endogenous reference gene zSSIIb could be amplified simultaneously in one PCR reaction.

关 键 词：转基因生物 抗虫玉米 转化体特异性PCR方法 

分 类 号：TS513[轻工技术与工程—皮革化学与工程]