多胺和乙烯对莴苣种子根侧根原基发生的影响  

The Effects of Exogenous Polyamines and Ethylene on the Formation of LRP of Lettuce Seed

作　　者：曹丽君[1] 王鑫昕[2] 

机构地区：[1]唐山师范学院,河北唐山063000 [2]邯郸学院,河北邯郸056005

出　　处：《安徽农业科学》2010年第22期11722-11724,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究多胺和乙烯对莴苣种子根侧根原基发生的影响。[方法]分别用不同浓度的Spm、Spd、Put、ACC、Ag+和Co2+配合IBA处理莴苣种子根,经Schiff法染色后观察侧根原基,以探讨外源多胺和乙烯对植物侧根发生的影响。[结果]100.0、1000.0μmol/L的腐胺(Put)和0.1μmol/L的亚精胺(Spd)对IBA诱导的侧根原基的发生有一定的叠加效应;但0.01～10.0μmol/L的精胺(Spm)均抑制IBA诱导的侧根原基的发生;ACC本身对侧根原基的发生没有作用,但ACC(1.0μmol/L)配合IBA(5.0μmol/L)处理后,可增强IBA对侧根原基的诱导作用。乙烯合成的抑制剂CoCl2(0.025～0.4μmol/L)和生理作用的抑制剂AgNO3(0.02～0.4μmol/L)均可减弱IBA诱导的侧根原基的发生。[结论]多胺和乙烯对IBA诱导的莴苣种子根的侧根原基发生具有协同的促进作用。[Objective]The aim was to research the effects of exogenous polyamines and ethylene on the formation of lateral root primodia （LRP） of lettuce seed.[Method]The LRP were dealt with different Put of Spm,Spd,Put,ACC,Ag＋ and Co2＋ with IBA to observe LRP after the method of Schiff.[Result]The result showed that 100.0,1 000.0 μmol/L Put and 0.1 μmol/L Spd promote the occurrence of lateral root primodia （LRP） induced by IBA;However,Spm had an inhibitory effect on the occurrence of LRP by IBA with 0.01～10.0 μmol/L.Although ACC had no effect on the formation of LRP,1.0 μmol/L ACC had additive effect on the 5.0 μmol/L IBA induction on the formation of LRP.CoCl2 （0.025-0.4 μmol/L） and AgNO3 （0.02-0.4 μmol/L）could decrease the number of LRP induced by IBA.[Conclusion]Exogenous polyamines and ethylene had synergistic promoting effect on the occurrence of LRP of lettuce seed induced by IBA.

关 键 词：多胺 乙烯 莴苣 侧根 

分 类 号：S636.2[农业科学—蔬菜学]