影响益母草愈伤组织诱导的因素  

Factors Affecting Callus Induction of Leonurusn artemisia

作　　者：林超群[1] 杨晖[1] 赵鹂[1] 

机构地区：[1]丽水学院,浙江丽水323000

出　　处：《安徽农业科学》2010年第22期11769-11770,共2页Journal of Anhui Agricultural Sciences

基　　金：浙江省大学生科技创新项目

摘　　要：[目的]研究影响益母草愈伤组织诱导的因素。[方法]以益母草种子萌发的真叶为外植体,MS为基本培养基,研究了不同浓度的植物激素和蔗糖以及不同的培养方式和光照条件对益母草愈伤组织诱导的影响。[结果]培养基中2,4-D为0.5或1.0mg/L和6-BA为2.0mg/L时,益母草愈伤组织诱导率相对较高,可达93.3%;愈伤组织诱导率随培养基中蔗糖含量的增加而增加,在蔗糖含量达到30g/L时达到最高,为86.7%,超过30g/L时则下降;在3种培养方式中,固体培养的诱导率是液体培养的1.63倍;3种光照中,全光条件下的愈伤组织诱导率可达到92.8%,全暗条件下的愈伤组织诱导率仅有43.6%。[结论]培养基添加合理的植物激素组合、蔗糖,并采用合适的培养方式和光照条件有利于益母草愈伤组织的诱导。[Objective] The propose was to study the factors affecting callus induction of Leonurusn artemisia.[Method] With the true leaves which germinated from L.artemisia seeds as explants and the MS medium as the basic medium,the effects of the plant hormone and the sucrose with different concentration and the different culture methods and illumination conditions on the callus induction rate of L.artemisia were studied.[Result] When 2,4-D was 0.5 or 1.0 mg/L and 6-BA was 2.0 mg/L in the medium,the callus induction rate of L.artemisia were higher,with the highest as 93.3%.The callus induction rate was increased with the increment of sucrose content in the medium,it got highest when the sucrose content reached to 30 g/L and it could be decreased when the sucrose content surpassed 30 g/L.Among 3 kinds of culture modes,the callus induction rate from the solid culture was 1.63 times of that from the liquid culture.Among 3 kinds of illumination,the callus induction rate under whole light could be up to 92.8%,while that under whole dark was only 43.6%.[Conclusion] Adding the rational plant hormone combination and sucrose and adopting the suitable culture mode and the illumination condition could be propitious to the induction of L.artemisia callus.

关 键 词：益母草 愈伤组织诱导 植物激素 蔗糖 培养方式 光照条件 

分 类 号：R282.71[医药卫生—中药学]