台湾甜象草的核型分析及rDNA荧光原位杂交  

Karyotype Analysis and Ribosomal DNA Fluorescent in Situ Hybridization in Pennisetum purpureum

作　　者：赵丽娟[1] 李立家[2] 

机构地区：[1]怀化学院生命科学系,湖南怀化418000 [2]武汉大学生命科学学院植物发育生物学教育部重点实验室,湖北武汉430072

出　　处：《安徽农业科学》2010年第22期12170-12173,共4页Journal of Anhui Agricultural Sciences

基　　金：湖南省杰出青年基金(30270678);国家自然科学基金资助项目(30270740)

摘　　要：[目的]对台湾甜象草的染色体进行识别并对该物种基因组的结构及进化进行研究。[方法]利用改进的火焰干燥法及荧光原位杂交技术,对台湾甜象草中期染色体的长度、着丝粒的位置及随体的数目进行分析,建立了台湾甜象草的经典核型。并根据各条染色体对4,6-二氨基-2-苯基吲哚(DAPI)染色的深浅度不同,参考DAPI对核物质的染色特点,了解各染色体上异染色质和常染色质的分布特点。[结果]利用荧光原位杂交技术检测了rDNA定位,得到2对45SrDNA的信号,分布在不同的染色体上,一对5SrDNA信号,位于9号染色体的长臂。确定了台湾甜象草的核型是2n=28=10sm+12m(4SAT)+4st+2T,为2C。[结论]为染色体的识别提供理论依据。[Objective]The aim of the study was to identify the chromosomes of Pennsetum purpureum,and know the genome structure and evolution.[Method] Mitotic metaphase chromosomes of Pennsetum purpureum were prepared using flame-drying method and fluorescent in situ hybridization technology,a classic karyotype of Pennsetum was constructed according to chromosome length,centromere position and the number of satellite.Kymogram of mitotic metaphase chromosomes on the basis of the 4＇-6-diamino-2-phenylindole dihydrochloride staining pattern was developed,and the distribution characteristics of heterochromatin in the Pennseturm purpureum genome was studied.[Result] By fluorescent analysis there were two pairs of 45S rDNA signals located on different chromosomes,and one pair of 5S rDNA signals mapped on the long arms of chromosome 9.The karyotype of the Pennsetum purpureum was 2n=28=10sm＋12m（4SAT）＋4st＋2T,it belonged to 2C.[Conclusion] The study provided theoretical basis for identification of the choromosomes.

关 键 词：台湾甜象草 荧光原位杂交 核型 

分 类 号：Q322[生物学—遗传学]