仔猪大肠埃希氏菌、C型产气荚膜梭菌的灭活及脱毒方法研究  

Inactivation and Detoxification of Escherichia coli and C-type Clostridium wechii

作　　者：刘泽文[1] 邓均华[1] 杨克礼[1] 梁望旺[1] 伍锐[1] 段正赢[1] 周丹娜[1] 田永祥[1] 徐涤平[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,湖北武汉430064

出　　处：《安徽农业科学》2010年第20期10702-10703,10728,共3页Journal of Anhui Agricultural Sciences

基　　金：湖北省科技厅引导项目(2006AA201C25);湖北省农业科技创新中心资助项目(2007-620-001-03)资助

摘　　要：[目的]研究仔猪大肠埃希氏菌病、C型产气荚膜梭菌病二联灭活疫苗的质量及产品的安全性。[方法]对大肠埃希氏菌和C型产气荚膜梭菌浓缩菌液,用不同浓度甲醛溶液经不同灭活时间、灭活温度进行了灭活和脱毒试验。[结果]大肠埃希氏菌培养物在37～38℃条件下用0.4%的甲醛溶液灭活48h,可以使细菌全部灭活;C型产气荚膜梭菌培养物在37～38℃,采用0.5%～0.8%的甲醛溶液进行灭活和脱毒7～14d,或者采用2次灭活和脱毒方法,7d可以达到完全灭活和脱毒。[结论]在37～38℃条件下,用0.4%的甲醛溶液对大肠埃希氏菌培养物灭活和采用0.5%～0.8%的甲醛溶液对C型产气荚膜梭菌进行灭活和脱毒,可以达到理想效果。[Objective] In order to assure the quality and security of Escherichia coli and C-type clostridium wechii combined vaccine.[Method] The different inactive temperature and time were tested on Escherichia coli and Clostridium wechii in different concentration formaldehyde.[Result] Escherichia coli was killed in 0.4% formaldehyde at 37-38 ℃ for 48 h.Clostridium wechii was inactived and losing virulence in 0.5%-0.8% formaldehyde at 37-38 ℃ for 7-14 d.[Conclusion] It can get better effect to Escherichia coli in 0.4% formaldehyde at 37-38 ℃ for 48 h and 0.5%-0.8% formaldehyde at 37-38 ℃ for 7-14 d to C-type Clostridium wechii.

关 键 词：大肠埃希氏菌 C型产气荚膜梭菌 灭活 脱毒 

分 类 号：S858.28[农业科学—临床兽医学]