几种不同信号通路的抑制剂对17β-雌二醇诱导绵羊输卵管上皮细胞β-防御素-1(SBD-1)基因转录的影响  

Effect of Several Different Signaling Pathway Inhibitors on Induction of β-Defensin-1(SBD-1) Gene Expression by 17β-Estrodiol in Epithelial Cells of Fallopian Tubes in Sheep

作　　者：包图雅[1,2] 曹贵方[1] 曹金山[1] 杜晨光[1] 凃勇[1] 

机构地区：[1]内蒙古农业大学动物胚胎与发育生物学研究室,呼和浩特010018 [2]内蒙古医学院基础医学院,呼和浩特010059

出　　处：《内蒙古大学学报（自然科学版）》2010年第4期398-403,共6页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金资助项目(No.30460093)

摘　　要：主要探讨了17β-雌二醇(E2)促进绵羊输卵管上皮细胞SBD-1(sheep beta-defensin-1)表达的可能的信号通路.在研究过程中,分别用10-8mol/L 17β-雌二醇及雌激素受体拮抗剂ICI182780、PKA(protein kinase A)阻断剂H-89、PKC(protein kinase C)阻断剂H-7、NF-κB(Nuclear factor-kappaB)阻断剂PDTC(pyrrolidine dithiocarbamate)干预绵羊输卵管上皮细胞6 h,采用实时荧光定量RT-PCR(reverse transcription polymerase chain reaction)方法检测SBD-1 mRNA表达水平的变化.实验结果显示,10-8mol/L 17β-雌二醇可显著促进绵羊输卵管上皮细胞SBD-1 mRNA的表达(p<0.01);ICI182780、H-89、PDTC和H-7均可阻断17β-雌二醇对SBD-1的上调作用.提示17β-雌二醇促进绵羊输卵管上皮细胞SBD-1表达是由雌激素核受体、PKA、NF-κΒ、PKC信号转导通路所介导的.To investigate the possible signaling pathways involved in the promoting effect of 17β-estrodiol on β-Defensin-1（SBD-1） in epithelial cells of fallopian tubes in sheep,the epithelial cells of fallopian tube of sheep were exposed to 17β-estrodiol（10-8 mol/L） with or without estrogen nuclear receptor antagonist ICI182780,PKC antagonist H-7,PKA antagonist H-89,nuclear factor kappa B antagonist PDTC for 6 h.Quantitative real time RT-PCR was used to detect the changes of SBD-1 mRNA.The expression of SBD-1 mRNA was up-regulated by 17β-estrodiol（10-8 mol/L）,while this effect was abrogated by ICI182780,H-89,H-7 and PDTC.These results suggest that 17β-estrodiol induces SBD-1 mRNA transcription in epithelial cells of fallopian tubes,through the pathways involving estrogen nuclear receptor,PKA,PKC and NF-κΒ.

关 键 词：17Β-雌二醇 输卵管上皮细胞 绵羊 SBD-1 信号通路 

分 类 号：Q756[生物学—分子生物学]