膜荚黄芪苯丙氨酸解氨酶特性的初步研究  

Preliminary study on the characteristics of phenylalanine ammonia-lyase of Astragalus membranaceus

作　　者：孙晓丹[1] 金美玉[1] 严一字[1] 全雪丽[1] 吴松权[1] 

机构地区：[1]延边大学农学院,吉林龙井133400

出　　处：《辽宁林业科技》2010年第5期1-2,35,共3页Liaoning Forestry Science and Technology

基　　金：国家自然科学基金资助项目(30860036)

摘　　要：该文初步研究了荚膜黄芪苯丙氨酸解氨酶(PAL)的基本特性。结果表明:PAL的最适反应时间为30min,PAL在Tris-HCl缓冲液中适宜的pH值为8.0,最适反应温度为37℃,PAL酶浓度过高或过低不利于酶的活力,PAL最适底物浓度为2mmol/L。The basic characteristics of phenylalanine ammoma-lyase （PAL） of Astragalus membranaceus was studied. The results showed that the optimum reaction time was 30 min, the optimum pH in Tris-HCl buffer was 8.0, the optimum reaction temperature was 37 ℃, higher or lower concentration of PAL was not approprite for the PAL activity, and the optimum substrate concentration of PAL was 2 mmol/L.

关 键 词：膜荚黄芪 苯丙氨酸解氨酶 特性 

分 类 号：Q552.3[生物学—生物化学]