牦牛睾丸组织能量代谢相关酶活力的分析  

Analysis of Energy Metabolism-related Enzyme Activities in Yak Testis Tissue

作　　者：邱翔[1] 林亚秋[1] 王国生[1] 阮武勇[1] 刘薇[1] 王翠丽[1] 黄林[1] 郑玉才[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《四川动物》2010年第5期590-592,F0002,共4页Sichuan Journal of Zoology

基　　金：国家自然科学基金(30972160);国家973计划前期研究专项(2007CB116204)的部分资助

摘　　要：为了解牦牛Bos grunniens睾丸组织在性成熟前后与能量代谢相关的几种酶活力变化,对九龙牦牛睾丸组织中参与糖酵解、三羧酸循环、脂肪酸β-氧化和线粒体呼吸链中几种酶活力做了测定。结果显示,性未成熟的九龙牦牛睾丸组织中β-羟脂酰CoA脱氢酶(HAD)活力显著高于成年九龙牦牛(P<0.01),而乳酸脱氢酶(LDH)、异柠檬酸脱氢酶(ICDH)、细胞色素氧化酶(COX)活力未见显著差异,提示脂肪酸β-氧化在性成熟前的牦牛睾丸组织能量代谢中可能发挥重要作用。The objective of the present study was to reveal activities of a few enzymes related to energy metabolism in immature and mature testes of the yak（Bos grunniens）.Analysis of a few enzymes（involved in glycolysis,citric acid cycle,fatty acid β-oxidation,and mitochondrial electron transfer chain）showed that Jiulong yak immature testes contained significantly higher activity of β-hydroxyacyl-CoA dehydrogenase（HAD）than mature testes（P〈0.01）,while no significant changes in activities of lactate dehydrogenase（LDH）,isocitrate dehydrogenase（ICDH）,and cytochrome c oxidase（COX）were observed.The results suggest that fatty acid β-oxidation may play an important role in energy metabolism of immature yak testes.

关 键 词：牦牛 睾丸 乳酸脱氢酶 细胞色素氧化酶 β-羟脂酰CoA脱氢酶 

分 类 号：S823[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]