大鼠耳蜗血管纹胆碱能M受体的表达及意义  

Expression of Cholinergic Muscarinic Receptor in Stria Vascularis of Rat Cochlea

作　　者：李隽[1,2] 孔维佳[1] 韩月臣[1] 程华茂[1] 胡钰娟[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,湖北武汉430022 [2]武汉市妇女儿童医疗保健中心耳鼻咽喉科,湖北武汉430016

出　　处：《江汉大学学报（自然科学版）》2010年第3期82-84,共3页Journal of Jianghan University：Natural Science Edition

摘　　要：目的:研究胆碱能M受体在耳蜗血管纹的表达.方法:采用RT-PCR技术检测大鼠耳蜗血管纹上胆碱能M受体的基因表达.结果:扩增出标志M2、M3、M4和M5受体亚型基因表达的PCR产物,未扩增出标志M1受体亚型的产物.结论:胆碱能M2、M3、M4和M5受体亚型在耳蜗血管纹有表达,提示胆碱能M受体可能参与了血管纹功能的调节,为研究胆碱能M受体在血管纹上的功能提供分子生物学基础.Objective：To examine the expression of cholinergic muscarinic receptor in the stria vascularis of rat cochlea. Methods： The gene expression of cholinergic muscarinic receptor in the stria vascularis of rat choclea was detected by RT-PCR. Results： RT-PCR of total RNA isolated from the stria vascularis of rat cochlea using primers specific for M2, M3, M4 and M5 muscarinic acetylcholine receptor subtypes revealed products, respectively. No appropriate product was found in ractions performed with primers specific for M1 muscarinic acetylcholine receptor subtype. Conclusion： The stria vascularis of rat cochlea contains transcripts for M2, M3, M4 and M5 muscarinic acetylcholine receptor subtypes, indicating that the muscarinic receptors may be involved in the regulation of the function of stria vascularis.

关 键 词：血管纹 胆碱能M受体 逆转录聚合酶链式反应 大鼠 

分 类 号：R76[医药卫生—耳鼻咽喉科] Q599[医药卫生—临床医学]