HPLC测定芙黄膏中大黄酸、大黄素的含量  

Determination of Rhein and Emodin in Fu Huang-Creamby HPLC

作　　者：缪婧[1] 许彦[1] 李福如[1] 葛玉珠[2] 

机构地区：[1]南通市中医院,江苏南通226001 [2]南京中医药大学,江苏南京210046

出　　处：《亚太传统医药》2010年第9期10-12,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:建立测定芙黄膏中大黄酸、大黄素含量的高效液相色谱法。方法:采用高效液相色谱法,以甲醇-0.1%磷酸溶液(85:15)为流动相;流速1.0mL/min;检测波长为254nm;柱温30℃。结果:大黄酸、大黄素分别在0.596～14.9μg/mL(r=0.9998)、0.32～8.0μg/mL(r=0.9997)范围内线性关系良好;平均回收率分别为99.1%、99.75%。结论:该法简便、准确、重复性好,可用于芙黄膏中大黄酸、大黄素的含量测定。Objective：To establish HPLC method for determination of rhein and emodin in Fu Huang-cream.Methods：Using HPLC.We used Methanol-0.1% phosphoric acid solution（85：15） as mobile phase; the fiow rate was 1.0mL/min and UV detection wavelength as 254nm; column temperature was 30℃.Results：Rhein and emodin were linear over the range of 0.596-14.9μg/mL （r=0.9998） and 0.32-8.0μg/mL （r=0.9997）.The average recovery were 99.1% and 99.75%.Conclustion：This method is simple, accurate and reproducible.It is suitable for determination of rhein and emodin in Fu Huang-cream.

关 键 词：芙黄膏 大黄酸 大黄素 HPLC 含量测定 

分 类 号：R284[医药卫生—中药学]