新城疫病毒F基因重要功能区和HN基因单核苷酸多态性研究  

Single Nucleotide Polymorphism of Functionally Important Region in F Gene and HN Gene of Newcastle Disease Virus

作　　者：张晓东[1] 刘怀然[1] 刘培欣[1] 刘胜旺[1] 孔宪刚[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室,黑龙江哈尔滨150001

出　　处：《中国家禽》2010年第16期14-17,共4页China Poultry

基　　金：国家科技支撑计划(2006BAD06A03、2006BAD06A08)

摘　　要：对广泛应用的新城疫疫苗株La Sota进行了全基因组测序,并对来自9个不同基因型新城疫病毒株的血凝素神经氨酸酶(haemagglution-neuraminidase,HN)基因和融合蛋白(fusion protein,F)基因47～435 nt区域进行了用单核苷酸多态性(SNP)分析。结果表明:在F基因47～435 nt间和HN基因中都存在高密度的SNP位点,Tajima′s D检验结果表明这些核苷酸变化符合中性突变假说,Pi(a)/Pi(s)分析暗示F蛋白编码基因N端的一些区段和HN蛋白编码基因中散在的部分区域受到了正向选择,但F基因和HN基因在整体上却都受到了强大的负向选择压力的影响。The whole genome of Newcastle disease virus vaccine strain La Sota which is widely used in practice was sequenced.Subsequently,sequence data of strains from nine genotypes were analyzed for single nucleotide polymorphism(SNP).The result revealed high frequency of SNP both in fusion(F) gene of 47 to 435 nt and hemagglutinin-neuraminidase(HN) gene.There was no significance of nucleotide diversity tested by Tajima′s D,indicating that neutral mutation hypothesis could explain the nucleotide polymorphism there.However,some regions in N-terminal of F gene and sporadic regions in HN gene could be subject to positive Darwin selection judged by Pi(a)/Pi(s)analysis,but whole F gene and HN gene were subject to strong negative selection.

关 键 词：单核苷酸多态性 HN基因 新城疫病 功能区 蛋白编码基因 全基因组测序 神经氨酸酶 融合蛋白 

分 类 号：S852.659.5[农业科学—基础兽医学]