凉山彝族自治州HIV流行毒株基因亚型分析  

Analysis of the HIV subtypes in Liangshan Yi Autonomous region

作　　者：计云霞[1] 白国刚[2] 刘志英[1] 吴亚松[1] 张彤[1] 苦约哈 瓦其车久 马英 徐萌[1] 陈德喜[1] 吴昊[1] 

机构地区：[1]首都医科大学附属北京佑安医院性病艾滋病实验室,100069 [2]解放军第309医院 [3]凉山彝族自治州昭觉县疾病预防控制中心

出　　处：《北京医学》2010年第9期730-733,共4页Beijing Medical Journal

基　　金：艾滋病急性期控制与治疗研究(D09050703590901);国家自然科学基金(30872226;30770742)

摘　　要：目的对16例来自凉山彝族自治州的HIV感染者进行亚型分析,监测该地区感染人群HIV毒株的流行情况。方法从16例HIV-1抗体阳性样本中分离外周血单核细胞(PBMC),从PBMC中提取基因组DNA,直接进行巢式PCR扩增HIVenv基因c2～v5区及gag基因p17～p24区,对PCR产物进行核苷酸序列测定和分析,确定基因亚型。结果 16例样本全部为BC重组亚型,env基因的基因距离较gag基因远,gag基因在进化上与CRF07-BC更为接近。结论凉山彝族自治州的HIV流行毒株主要为CRF07-BC。Objective To monitor the epidemiology of HIV by analyzing HIV subtype of 16 subjects with HIV-1 in the Liangshan Yi autonomous region.Methods DNA was extracted from the PBMCs of 16 HIV-1 positive samples,HIV env（c2～v5 region） and gag（p17～p24） gene were amplified by nested-PCR using specific primer pairs and the product was sequenced directly.The acquired sequences were analyzed to determine the HIV subtype.Results All the cases were the B/C recombinant strains,the gene distance of env region is farther than that of gag region.Liangshan Yi Autonomous Region is CRF07-BC,and the prevalence of HIV-1 among the cases,phylogenetic analysis showed that gag gene of all the cases were closer to CRF07 -BC than CRF08 -BC.Conclusions The major subtype of HIV -1 among IDUs in Yi population in Liangshan is CRF07-BC.The transmition of HIV-1 tends from the IDUs to common population in this region now.

关 键 词：凉山彝族人群 人类免疫缺陷病毒1型 亚型 系统进化树分析 

分 类 号：R512.91[医药卫生—内科学]