异甘草素对氢过氧化枯烯所致神经母细胞瘤损伤的影响  

Effect of Isoliquiritigenin on the Cumenehydroperoxide-induced Damages in SH-SY5Y Human Neuroblastoma Cells

作　　者：张玉柯[1] 崔誉蓉[1] 陈朋[1] 王振华[1] 郑秋生[1] 

机构地区：[1]省部共建新疆特种植物药资源教育部重点实验室.石河子大学药学院,新疆石河子832002

出　　处：《时珍国医国药》2010年第9期2159-2161,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30860074);教育部新世纪优秀人才支持项目(No.NCET-06-0918);新疆生产建设兵团博士资金项目(No.ZD2007JC04);新疆生产建设兵团基础研究计划项目(No.2007JC03)

摘　　要：目的研究异甘草素(ISL)对氢过氧化枯烯(cumenehydroperoxide,CHP)诱导人神经母细胞瘤SH-SY5Y氧化损伤的影响。方法 ISL处理体外培养的SH-SY5Y细胞24h后,添加不同浓度的CHP继续培养24h,进行细胞形态观察、细胞活力和细胞凋亡检测、细胞内活性氧(ROS)水平和丙二醛(MDA)含量测定。结果 CHP引起并促进人神经母细胞瘤SH-SY5Y氧化损伤,ISL可拮抗CHP所致的细胞的凋亡,降低CHP所致的ROS水平和MDA含量的增高。结论 ISL抑制脂质过氧化物的产生并降低细胞凋亡率,对人神经母细胞瘤SH-SY5Y氧化损伤有保护作用。Objective To study the effect of isoliquiritigenin on cumenehydroperoxide-induced oxidative damages in SH-SY5Y human neurobastoma cells.MethodsThe SH-SY5Y cells were pretreated with isoliquiritigenin for 24h,then treated by different concentrations of cumenehydroperoxide.Cell morphology was observed,neuronal apoptosis rate and survival rate were determined,the contents of ROS and malondialdehyde（MDA）were measured.ResultsCumenehydroperoxide was able to cause oxidative damage in SH-SY5Y cells.Isoliquiritigenin decreased the neuronal apoptosis rate,reduced MDA content and ROS level induced by cumenehydroperoxide.ConclusionIsoliquiritigenin can restrain the lipid peroxidation and neuronal apoptosis rate induced by oxidative stress.It has the protective effects against cumene hydroperoxide-induced oxidative damages in SH-SY5Y human neurobastoma cells.

关 键 词：异甘草素 人神经母细胞瘤SH-SY5Y 氢过氧化枯烯 氧化损伤 

分 类 号：R285.5[医药卫生—中药学]