薏苡非种仁部位甲醇提取物的指纹图谱研究  

Chromatogram by Fingerprint of Coix lacryma-jobi L. var. mayuen (Roman.) Stapf

作　　者：李厚聪[1] 袁玮[2] 李莹[2] 刘圆[2] 

机构地区：[1]湖北民族学院.医学院,恩施湖北445000 [2]西南民族大学少数民族药物研究所,成都四川610041

出　　处：《时珍国医国药》2010年第9期2174-2176,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技支撑计划重点项目(No.2007BAI25B05);四川省青年基金项目(No.07ZQ026-011);国家中医药管理局项目(No.06-07ZP43)

摘　　要：目的建立薏苡药材茎中甲醇部位的RP-HPLC指纹图谱的研究方法。方法采用Kromasil C18色谱柱(Dikma公司,4.6mm×250mm,5μm),以甲醇-水为流动相梯度洗脱,流速1ml.min-1,柱温30℃,检测波长263nm。结果用梯度洗脱得到的色谱图各色谱峰分离较好,达到指纹图谱要求。10个产地薏苡药材共标定了12个共有峰。结论该方法简便、实用、可靠,可以作为评价薏苡药材质量的基础。Objective To establish reversed-phase high performance liquid chromatography（RP-HPLC）for determination of fingerprint of C.lacryma-jobi L.var.mayuen（Roman.）Stapf.Methods The sample was separated on a Kromasil C18（4.6 mm ×250 mm,5 μm）column with gradient elution.The mobile phase consisted of methol and water.The flow rate was 1.0 ml·min-1 ;the temperature of column was at 30 ℃;UV detection wavelength was at 263 nm,and recording time was 75 min.ResultsHPLC chromatogram by Fingerprint of C.lacryma-jobi L.var.mayuen（Roman.）Stapf was obtained.12 common peaks in the HPLC profile were found in C.lacryma-jobi L.var.mayuen（Roman.）Stapf in 10 different growing areas.ConclusionThis method is simple,practicable and reliable and can be used for the quality control of C.lacryma-jobi L.var.mayuen（Roman.）Stapf.

关 键 词：薏苡 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]