黄芪药材中黄芪甲苷含量的荧光分光光度法测定  

Determination of Astragaloside IV in Radix Astragali by Spectrophotofluorimetry

作　　者：杨连梅[1,2] 刘养清[2] 赵慧辉[3] 李桂兰[2] 胡荣 

机构地区：[1]扬州大学,江苏扬州225001 [2]山西中医学院,山西太原030024 [3]北京中医药大学,北京100029

出　　处：《时珍国医国药》2010年第9期2272-2273,共2页Lishizhen Medicine and Materia Medica Research

基　　金：山西省国际科技合作项目(No.2007081032)

摘　　要：目的建立荧光分析法测定黄芪药材中黄芪甲苷的含量方法。方法利用大茴香酸在硫酸介质中与黄芪甲苷反应产生强烈荧光的特性进行测定黄芪中黄芪甲苷含量。结果最大激发波长Ex为320nm,最大发射波长Em为387nm。测定样品的平均回收率为97.22%,RSD=2.31%(n=5)。结论该方法灵敏度高,选择性好,结果无干扰,可广泛应用于黄芪药材的质量控制。Objective To determine the content of Aragaloside IV in Radix Astragali by spectrophotofluorimetry.MethodsThe fluorescence characteristics of astragaloside anisic reaction product were used to dertermine aragaloside IV by spectrophotofluorimetr.ResultsThe reaction product of astrogaloside and anisic acid had excitation and emission maxima at 320and 387nm,respectively.The recovery of measurement was 97.22%（RSD =2.31,n=5）.ConclusionThe method has advantages of high sensitivity,low detection limit and no interference.It also can be used for quality control of Aragaloside IV in Radix Astragali.

关 键 词：荧光分光光度法 黄芪 黄芪甲苷 

分 类 号：R284.2[医药卫生—中药学]