硅锆复合氧化物的制备、表征及其在磷酸化肽段富集中的应用  

Synthesis and Characterization of Silica-Zirconia Composite for Enrichment of Phosphopeptides

作　　者：吴剑虹[1] 肖矿[1] 赵勇 章伟平[2] 郭林[2] 冯钰锜[1] 

机构地区：[1]武汉大学化学与分子科学学院,生物医学分析化学教育部重点实验室,武汉430072 [2]武汉大学化学与分子科学学院,生命科学学院,武汉430072

出　　处：《分析化学》2010年第9期1231-1237,共7页Chinese Journal of Analytical Chemistry

基　　金：国家科技部973计划(No2007CB914200)资助项目

摘　　要：采用溶胶-凝胶法制备了氧化硅-氧化锆复合氧化物(SiO2-ZrO2),并对其物理与化学性质进行了详细地表征。以α-酪蛋白的酶解产物为探针,比较了氧化硅-氧化锆复合氧化物与氧化锆对磷酸化肽段的富集能力。结果表明:在pH<2时富集,SiO2-ZrO2对磷酸化肽的选择性要优于ZrO2;使用SiO2-ZrO2材料时,多磷酸化肽段的回收率更高。结合MALDI-TOFMS检测技术,SiO2-ZrO2成功地应用于脱脂牛奶酶解产物中磷酸化肽的分离和富集。Silica-zirconia composite（SiO2-ZrO2） was synthesized by sol-gel method and its physical-chemical properties were investigated with several techniques.The enrichment capacity of silica-zirconia composite and zirconia was compared using tryptic digest of α-casein as probe.The results showed that the selectivity of SiO2-ZrO2 was better than that of ZrO2 when pH value of loading solution was 2.The enrichment capacity of SiO2-ZrO2 for multiple phosphorylated peptides was better than that of ZrO2.In combination with MALDI-TOF based peptide mass finger printing technique,SiO2-ZrO2 was used to enrich the phosphopeptides from nonfat milk successfully.

关 键 词：氧化硅-氧化锆复合氧化物 磷酸化肽段 富集 基质辅助激光解吸飞行时间质谱 

分 类 号：Q50[生物学—生物化学]