多孔“类碳糊电极”的羧基化及其对Escherichia ocli O157:H7的检测  

Carboxylation of Porous Pseudo-carbon Paste Electrode and Its Application to Detection of Escherichia coli O157:H7

作　　者：许利剑[1,2,3] 杜晶晶[3] 邓燕[1,3] 李智洋[1] 徐春祥[1,2] 何农跃[1,3] 汪华[4] 史智扬[4] 

机构地区：[1]东南大学生物电子国家重点实验室,南京210096 [2]东南大学电子科学与工程学院,南京210096 [3]湖南工业大学绿色包装与生物纳米技术应用实验室,株洲412008 [4]江苏省疾病预防控制中心卫生部肠道病原微生物重点实验室,南京210009

出　　处：《分析化学》2010年第9期1261-1266,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos60927001;60971045);国家科技重大专项(No2009ZX10004-311);国家重大科学研究计划(No2010CB933903);国家"863"重点项目(No2007AA022007);中国博士后科学基金(No20090461057);江苏省博士后科研资助计划(No0902048C);湖南省自然科学基金(Nos09JJ3017;10JJ4010);江苏省医学重点实验室肠道致病菌实验室开放课题资助

摘　　要：以吡咯为前驱体,羧基化碳纳米管、石墨粉为填料和碳酸钙微球为模板直接诱导合成,制备出一种高灵敏的多孔"类碳糊电极"生物电化学传感器,讨论了羧基化碳纳米管含量、银染时间对检测结果的影响。结果表明,最佳羧基化碳纳米管含量为8%,最佳银染时间为12min,银的阳极溶出峰电流与E.ocliO157:H7浓度在1.0×104～1.0×106cells/mL范围内呈线性关系,其线性回归方程为:IP=-5.582+1.972logC,相关系数(R2)为0.9912,检出限为5.1×103cells/mL,实现了对E.ocliO157:H7快速、准确地检测。A novel and sensitive electrochemical biosensor based on porous pseudo-carbon paste electrode（PPCPE） was described.PPCPE was fabricated by mixing calcium carbonate microspheres as the template,graphite powders and carbon nanotubes as the fillers,pyrrole as the precursor of polymer which actually acted as the pastE.The effects of content of carbon nanotube functionalized with carboxyl group（NWCNT-COOH） in carboxylated of porous pseudo-carbon paste electrode（PPCPE-COOH） and the silver enhancement time were investigated.The results showed that the optimal contents of NWCNT-COOH was 8%,the optimal silver enhancement time was 12 min.A linear relationship between the silver anodic stripping peak current and the concentration logarithm of E.ocli O157：H7 from 1.0×104 to 1.0×106 cells/mL was obtained,with a correlation coefficient of 0.9912 and a detection limit of 5.1×103 cells/mL.

关 键 词：羧基化碳纳米管 多孔“类碳糊电极” 生物电化学传感器 金标银染 生物电子 大肠杆菌 

分 类 号：TH776[机械工程—仪器科学与技术]