毛细管电泳柱端电化学发光法分离测定药物和尿液中的盐酸维拉帕米  

Separation and Determination of Verapamil Hydrochloride by Capillary Electrophoresis with End-column Electrochemiluminescence Detection

作　　者：梁汝萍[1] 朱晓艳[1] 陶移文[2] 汪敬武[1] 

机构地区：[1]南昌大学化学系,南昌330031 [2]广州医学院药学系,广州510182

出　　处：《分析化学》2010年第9期1305-1310,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No20805023);江西省教育厅科技项目基金(NoGJJ10014)资助项目

摘　　要：采用带有柱端电化学发光(ECL)检测池的毛细管电泳(CE)法分离并测定盐酸维拉帕米(Verapamil,VRPM)。盐酸VRPM作为共反应剂,增强了吡啶钌的电化学发光强度。在优化的条件下,ECL强度与VRPM在7.0×10-7～5.0×10-4mol/L范围内呈良好的线性关系;检出限(S/N=3)为4.6×10-8mol/L。本方法已成功用于药物和人体尿样中盐酸VRPM含量的测定。研究了VRPM与牛血红蛋白(BHb)的相互作用,计算其结合常数为3.72×103L/mol,最大结合量为1.375×10-4mol。Based on the fact that verapamil can increase tris（2,2＇-bipyridyl） ruthenium（Ⅱ） [Ru（bpy）32＋] electrochemilumines,a capillary electrophoresis method with end-colum electrochemiluminescence detection was developed for the detection of verapamil hydrochloride in pharmaceuticals and human urine.Under optimized conditions,the ECL intensity was linear with the concentration of verapamil over the range from 7.0×10-7 to 5.0×10-4 mol/L with a detection limit of 4.6×10-8 mol/L（S/N=3）.This method was success-fully applied to the detection of verapamil in pharmaceuticals and human urine.The interaction of verapamil with bovine hemoglobin was also investigated.The binding constant and the maximum binding capacity were calculated to be（K） 3.72×103 L/mol and（αmax） 1.375×10-4 mol,respectively.

关 键 词：毛细管电泳 柱端电化学发光 盐酸维拉帕米 吡啶钌 药物分析 

分 类 号：R927[医药卫生—药学] R965