微流控芯片光学检测技术在细胞研究中的应用与进展  

Recent Advances on Optical Detection Methods and Techniques for Cell-Based Microfluidic Systems

作　　者：霍丹群[1] 刘振[1] 侯长军[1] 杨军[1] 罗小刚[1] 法焕宝[2] 董家乐[1] 张玉婵[1] 张国平[1] 李俊杰[1] 

机构地区：[1]重庆大学生物工程学院生物流变科学与技术教育部重点实验室,重庆400044 [2]重庆大学化学化工学院,重庆400044

出　　处：《分析化学》2010年第9期1357-1365,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos30770568;30770569);国家"863"项目(No2006AA04Z349);重庆大学研究生创新团队(No200909B1008)资助

摘　　要：重点综述了芯片上细胞培养和实时检测的微系统研究及相应检测方法和技术近年来的进展。总结了多种光学检测方法和技术体系的优缺点及发展趋势,讨论了各种方法技术对细胞研究模型的研究适用范围,探讨各种检测方法和技术对活细胞、单细胞实时无损无标记检测和传感发展前景和研究进展。最后对整个芯片实验室框架下细胞研究及传感微系统的发展方向进行了有益的思路和方法展望。Recent advances on optical detection methods and techniques applied in cell-based microfluidic systems are reviewed.Microsystems that integrate on chip cell culture and real-time detection,and recent advances on correlated detection methods and techniques are mainly focused.Comparison of six optical detection methods and techniques are summarized,and their research applicability for cell study is proposed.Trend and development perspective of each technique applied for living cell label-free and real-time detection and sensing are discussed.Finally,an assessment of future directions for lab chip based cell research and sensing microsystem are concluded.

关 键 词：微流控芯片 细胞研究 光学检测 综述 

分 类 号：Q2-3[生物学—细胞生物学] TN492[电子电信—微电子学与固体电子学]