弓形虫代谢分泌抗原对仔猪IL-2、IL-4、IFN-γ和TNF-α表达水平的影响  

Effects of Toxoplasma gondii excreted-secreted antigens on the expression level of IL-2,IL-4,IFN-γ and TNF-α in piglets

作　　者：蔡志杰[1,2] 王艳华[2] 李文卉[2] 芦赟[1,2] 张燕丽[1,2] 曹丽艳[1,2] 付宝权[2] 张德林[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,农业部兽医公共卫生重点开放实验室,甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《甘肃农业大学学报》2010年第4期10-15,共6页Journal of Gansu Agricultural University

基　　金：国家"十一五"科技支撑计划项目(2007BAD40B05);公益性行业(农业)科研专项经费项目(200803017);甘肃省重大专项科技项目(2GS063-A43-013)

摘　　要：通过免疫后攻毒的方法研究了弓形虫代谢分泌抗原(E/SA)免疫后对不同免疫组仔猪机体白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)含量的影响.结果表明:免疫后各免疫组仔猪的IL-2、IL-4、IFN-γ含量均不同程度的升高;其中E/SA组IL-2、IL-4和IFN-γ的水平显著升高,与对照组差异显著(P<0.05),其含量分别由免疫前的105.05、105.33、67.50pg.mL-1升高到免疫后的194.03、342.50、296.50pg.mL-1;TNF-α水平变化不显著.试验证实弓形虫代谢分泌抗原疫苗能够促进仔猪IL-2、IL-4、IFN-γ的分泌,并引起宿主抗弓形虫感染的免疫应答.The effects of the Toxoplasma gondii excreted-secreted antigens（E/SA）vaccine on piglet＇s IL-2,IL-4,IFN-γ and TNF-α were investigated.The piglets were randomly divided into 6 groups with 5 animals in each group,named E/SA group,E/SA＋CpG（unemulsified） group,E/SA＋CpG group,E/SA＋IL-2 group,E/SA＋IL-2＋CpG group and the control group.The animals were immunized twice at 2 weeks interval and the challenge infection with T.gondii was conducted 2 weeks after the second vaccination.The blood sera were collected before the first immunization,the second immunization,the challenge,1 and 2 weeks after the second immunization,respectively.The levels of IL-2,IL-4,IFN-γ and TNF-α were detected by ELISA.The levels of IL-2,IL-4,IFN-γ in immunized groups increased and the E/SA group increased significantly compared with the control group（P〈0.05）,from 105.05,105.33,67.50 pg·mL^-1 to 194.03,342.50,296.50 pg·mL^-1.TNF-α level did not change significantly at all time.These data indicated that Toxoplasma gondii E/SA vaccine can promote the secretion of IL-2,IL-4 and IFN-γ in piglets and cause the anti-T gondii infection immune response.

关 键 词：仔猪 刚地弓形虫 代谢分泌抗原 白细胞介素-2 白细胞介素-4 干扰素-Γ 肿瘤坏死因子-α 

分 类 号：S828[农业科学—畜牧学]