红芪、黄芪水提液体外清除自由基作用的研究  

Study on free radical scavenging activities of water extract from Hedysarum polybotrys and Astragalus membranaceus in vitro

作　　者：杨小虎[1] 郭延生[1] 曲亚玲[1] 华永丽[1] 杜天玺[1] 杨洪申[1] 魏彦明[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2010年第4期42-45,50,共5页Journal of Gansu Agricultural University

基　　金：甘肃省科技支撑计划项目(0804NKCA110)

摘　　要：采用Fenton反应建立羟基自由基(.OH)模型,利用邻苯三酚在碱性环境中自氧化产生超氧阴离子自由基(O2-.)模型,研究了红芪、黄芪水提液体外清除自由基的活性.结果表明:2种水提液在生药浓度为5～80mg.mL-1的剂量范围内对.OH的清除效果呈明显的量效依赖关系,其IC50分别为红芪水提液8.686mg.mL-1和黄芪水提液16.662mg.mL-1;二者在生药浓度为25～200mg.mL-1的剂量范围内对O2-.的清除效果呈现明显的量效依赖关系,其IC50分别为红芪水提液176.636mg.mL-1和黄芪水提液185.123mg.mL-1.红芪、黄芪水提液均具有一定的体外清除自由基活性,且红芪的清除效果优于黄芪,因此在以自由基为诱因的疾病防治中建议优先选用红芪.To research the free radical scavenging activities of water extracts from Hedysarum polybotrys（WEH） and Astragalus membranaceus （WEA） in vitro, the scavenging activities of WEA and WEH were compared using the hydroxyl radical system and superoxide anion free radical system. The results showed that both water extract had significant scavenging effects on hydroxyl radical from 5 mg·mL^-1 to 80 mg·mL^-1 in vitro. Furthermore, a significant concentration-dependent elimination was exhibited, the same tendency was showed when both extract on superoxide anion free radical from 25mg·mL^-1 to 200mg·mL^-1. The quality concentration IC50 was 16. 662mg·mL^-1 for the WEA to inhibit hydroxyl radical, while ICso for WEH was 8. 686 mg·mL^-1. The quality concentration IC50 was 185. 123 mg·mL^-1 for WEA to superoxide anion free radical,while IC50 for WEH was 176. 636mg·mL^-1. It is suggested that the scavenging capability of WEA to both free radical was weaker than WEH, therefore, water extract of Hedysarum polybotrys can be used for diseases linked with too many oxygen radicals.

关 键 词：红芪 黄芪 水提液 羟基自由基 超氧阴离子自由基 

分 类 号：R285.5[医药卫生—中药学]