双歧杆菌表面分子对LPS在小鼠体内调节胸腺细胞凋亡的观察  

Regulation of Bifidobacterium bifidum surface molecules on apoptosis of murine thymocytes induced by LPS in vivo

作　　者：余振东[1,2] 胡宏[1,2] 

机构地区：[1]同济医科大学附属协和医院检验科 [2]重庆医科大学检验系

出　　处：《中华微生物学和免疫学杂志》1999年第3期180-183,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金

摘　　要：目的研究双歧杆菌表面分子细胞壁肽聚糖（ＷＰＧ）、脂磷壁酸（ＬＴＡ）对ＬＰＳ体内诱导小鼠胸腺细胞凋亡的调节。方法用ＤＮＡ凝胶电泳、ＴＵＮＥＬ法检测ＷＰＧ、ＬＴＡ对ＬＰＳ体内诱导小鼠胸腺细胞凋亡的影响，并分别用生物活性法和Ｇｒｉｅｓｓ反应测定ＷＰＧ、ＬＴＡ、ＬＰＳ体外诱生ＴＮＦ－α、ＮＯ２－的含量。结果ＷＰＧ、ＬＴＡ可显著抑制ＬＰＳ体内诱导的小鼠胸腺细胞的凋亡。ＷＰＧ、ＬＴＡ单独刺激巨噬细胞时所诱生的ＴＮＦ－α、ＮＯ的量显著低于ＬＰＳ刺激时所产生的这两种活性介质的量，而ＷＰＧ、ＬＴＡ与ＬＰＳ共同应用时可显著降低ＬＰＳ诱导巨噬细胞产生的ＴＮＦ－α、ＮＯ的量；诱生型一氧化氮合成酶抑制剂Ｓ－甲基异硫脲硫酸盐体外可抑制巨噬细胞产生的ＮＯ的量，在体内可部分抑制ＬＰＳ诱导的小鼠胸腺细胞的凋亡。结论ＷＰＧ、ＬＴＡ与ＬＰＳ共同应用时，可抑制ＬＰＳ诱导的巨噬细胞产生的ＴＮＦ－α、ＮＯ的量。Objective To investigate the regulation of Bifidobacterium bifidum surface molecules whole peptidoglycan (WPG), lipoteichoic acid (LTA) on apoptosis of murine thymocytes induced by LPS in vivo. Methods Apoptosis of murine thymocytes in the cortex in vivo was analysed with methods of DNA gel electrophoresis and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). Results in vivo, the induction of apoptosis in murine thymocytes by LPS was significantly inhibited by WPG and LTA. in vitro, LPS induced significantly more TNF α and NO which released from murine macrophages than all of the tested surface molecules did. When WPG and LTA were combined with LPS respectively in stimulating murine macrophages, TNF α and NO release significantly decreased compared with that induced by LPS alone. SMT, a selective inhibitor of inducible nitric oxide synthase, not only decreased NO activity in murine macrophages induced by LPS in vitro, but also inhibited apoptosis of murine thymocytes induced by LPS in vivo. Conclusion The results suggest that WPG and LTA reduce LPS stimulated TNF α and NO release, then down regulate apoptosis of murine thymocytes induced by LPS in vivo.

关 键 词：双歧杆菌 表面分子 脂多糖 细胞凋亡 胸腺细胞 

分 类 号：R378.99[医药卫生—病原生物学]