中国钩端螺旋体rRNA基因限制性内切酶酶切位点多态性分类研究  

Identification of Leptospira in China by mapped restriction sites polymorphisms of rRNA gene

作　　者：冯晓峰[1,2] 秦进才[1,2] 梁中兴[1,2] 时曼华 王岳竹[1,2] 聂一新 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所 [2]中国药品生物制品检定所

出　　处：《中华微生物学和免疫学杂志》1999年第3期192-196,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的初步建立我国致病性钩端螺旋体ｒＲＮＡ基因限制性内切酶酶切位点多态性分类体系。方法采用ｒＲＮＡ基因限制性内切酶酶切位点多态性（ＭＲＳＰ）方法对６５个血清型７５株国内外钩端螺旋体参考株及２７株野生株进行分析。结果１６ＳｒＲＮＡ及２３ＳｒＲＮＡ基因在ＨｉｎｆⅠ、ＤｄｅⅠ的酶切位点上呈多态性，分别存在１８种和２４种不同的图谱。结合两种方法则有３４种不同的图谱。致病性钩端螺旋体间ＭＲＳＰ谱型差别相对较小，遗传距离较近，而它们与非致病性钩端螺旋体的ＭＲＳＰ谱型相差大，遗传距离远。国内主要流行型的ＭＲＳＰ只表现为ＭＲＳＰ１型和２型，非主要流行型的ＭＲ－ＳＰ型变化较多。大多数野生株与相应的参考株的ＭＲＳＰ型相同，个别具有不同ＭＲＳＰ型的野生株与相应参考株的遗传距离较近。结论ｒＲＮＡ基因限制性内切酶酶切位点多态性（ＭＲＳＰ）可用于钩体分类。Objective To establish an initial system of identification of Leptospira in China by mapped restriction sites polymorphisms of rRNA gene. Methods 75 Leptospira international and domestic reference strains, belonging to 64 serotypes, and 27 field strains were examined using HinfⅠ and DdeⅠ mapped restriction sites polymorphisms (MRSP) of rRNA gene. Results 18 and 24 MRSP profiles were observed in 16S rRNA and 23S rRNA gene, respectively, and the total MRSP profiles were 34. The profiles of pathogenic strains had less difference from each other but more difference from nonpathogenic strains. Only MRSP 2 and 1 were observed among prevalence strains of China, and there were varieties of MRSPs among the non prevalence strains. The profiles of most field strains are in correspondence with reference strains. Conclusions Restriction sites polymorphisms of rRNA gene (MRSP) for Leptospira can be used to classify and identify Leptospira in China and also to study on molecular epidemiology for leptospirosis.

关 键 词：钩端螺旋体 MRSP 基因分类 分类 

分 类 号：R377.5[医药卫生—病原生物学]